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Literature summary for 1.2.1.36 extracted from
- Recombinant class I aldehyde dehydrogenases specific for all-trans- or 9-cis-retinal (2002), J. Biol. Chem., 277, 17486-17492.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rattus norvegicus |
| functional expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of recombinant chimeric enzymes exchanging parts of the enzyme by sequences of the phenobarbital-induced aldehyde dehydrogenase from rat, and vice versa, the chimeric enzymes show altered substrate specificity towards 9-cis- or all-trans-retinal, overview | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, recombinant wild-type and chimeric mutant enzymes | Rattus norvegicus | |
| 0.0087 | - |
9-cis-retinal | 25°C | Rattus norvegicus |  |
| 0.0087 | - |
9-cis-retinal | recombinant wild-type enzyme, pH 7.2, 25°C | Rattus norvegicus | |
| 0.01 | - |
all-trans-retinal | 25°C | Rattus norvegicus | |
| 0.01 | - |
all-trans-retinal | recombinant wild-type enzyme, pH 7.2, 25°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| retinal + NAD+ + H2O | Rattus norvegicus | - |
retinoate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus | - |
class I enzyme | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| retinal + NAD+ + H2O = retinoate + NADH + H+ | catalytic active nucleophil Cys302, not responsible for substrate specificity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9-cis-retinal + NAD+ + H2O | - |
Rattus norvegicus | 9-cis-retinoate + NADH | - |
? | |
| 9-cis-retinal + NAD+ + H2O | poortly active with | Rattus norvegicus | 9-cis-retinoate + NADH | - |
? | |
| all-trans-retinal + NAD+ + H2O | - |
Rattus norvegicus | all-trans-retinoate + NADH | - |
? | |
| propanal + NAD+ + H2O | - |
Rattus norvegicus | propionate + NADH | - |
? | |
| retinal + NAD+ + H2O | - |
Rattus norvegicus | retinoate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PB-RALDH | - |
Rattus norvegicus |
| phenobarbital-induced aldehyde dehydrogenase | - |
Rattus norvegicus |
| RALDH | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
about, assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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