Cloned (Comment) | Organism |
---|---|
expression of isozyme type-1 in Escherichia coli strain BL21(DE3) as N-terminally fusion protein to glutathione-S-transferase | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetaldehyde | 60% competitive inhibition of all-trans-retinal oxidation at 0.4 mM | Mus musculus | |
Chloral hydrate | 80% competitive inhibition of all-trans-retinal oxidation at 0.1 mM | Mus musculus | |
Mg2+ | about 50% inhibition of all-trans- and 9-cis-retinal oxidation above 1 mM | Mus musculus | |
retinol | 40% uncompetitive inhibition of all-trans-retinal oxidation at 0.012 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
9-cis-retinal | recombinant isozyme type-1, pH 7.5, 25°C | Mus musculus | |
0.0116 | - |
all-trans-retinal | recombinant isozyme type-1, pH 7.5, 25°C | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, recombinant detagged isozyme type-1, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-cis-retinal + NAD+ + H2O | Mus musculus | - |
9-cis-retinoate + NADH | - |
? | |
all-trans-retinal + NAD+ + H2O | Mus musculus | - |
all-trans-retinoate + NADH | - |
? | |
additional information | Mus musculus | enzyme is involved in retinoic acids biosynthesis, which are important signaling molecules in embryogenesis and tissue differentiation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
isozyme type-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme type-1 as fusion protein from Escherichia coli by GST-affinity chromatography, cleavage by thrombin to eliminate the fusion tag | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-cis-retinal + NAD+ + H2O | - |
Mus musculus | 9-cis-retinoate + NADH | - |
? | |
all-trans-retinal + NAD+ + H2O | - |
Mus musculus | all-trans-retinoate + NADH | - |
? | |
all-trans-retinal + NAD+ + H2O | preferred substrate of recombinant isozyme type-1 | Mus musculus | all-trans-retinoate + NADH | - |
? | |
additional information | enzyme is involved in retinoic acids biosynthesis, which are important signaling molecules in embryogenesis and tissue differentiation | Mus musculus | ? | - |
? | |
additional information | substrate specificities at different substrate concentrations, no activity with 13-cis-retinal | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, recombinant detagged isozyme type-1, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
RalDH1 | - |
Mus musculus |
retinaldehyde dehydrogenase type-1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
recombinant isozyme type-1 exhibits 2 pH-optima at pH 7.8 and pH 9.4 with substrates all-trans-retinal and 9-cis-retinal, respectively | Mus musculus |
9.4 | - |
recombinant isozyme type-1 exhibits 2 pH-optima at pH 7.8 and pH 9.4 with substrates all-trans-retinal and 9-cis-retinal, respectively | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant isozyme type-1 | Mus musculus | |
0.005 | - |
Chloral hydrate | recombinant isozyme type-1, pH 7.5, 25°C, with substrate all-trans-retinal | Mus musculus | |
0.049 | - |
acetaldehyde | recombinant isozyme type-1, pH 7.5, 25°C, with substrate all-trans-retinal | Mus musculus |