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Literature summary for 1.2.1.36 extracted from

  • Gagnon, I.; Duester, G.; Bhat, P.V.
    Enzymatic characterization of recombinant mouse retinal dehydrogenase type 1 (2003), Biochem. Pharmacol., 65, 1685-1690.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozyme type-1 in Escherichia coli strain BL21(DE3) as N-terminally fusion protein to glutathione-S-transferase Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde 60% competitive inhibition of all-trans-retinal oxidation at 0.4 mM Mus musculus
Chloral hydrate 80% competitive inhibition of all-trans-retinal oxidation at 0.1 mM Mus musculus
Mg2+ about 50% inhibition of all-trans- and 9-cis-retinal oxidation above 1 mM Mus musculus
retinol 40% uncompetitive inhibition of all-trans-retinal oxidation at 0.012 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
9-cis-retinal recombinant isozyme type-1, pH 7.5, 25°C Mus musculus
0.0116
-
all-trans-retinal recombinant isozyme type-1, pH 7.5, 25°C Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, recombinant detagged isozyme type-1, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
9-cis-retinal + NAD+ + H2O Mus musculus
-
9-cis-retinoate + NADH
-
?
all-trans-retinal + NAD+ + H2O Mus musculus
-
all-trans-retinoate + NADH
-
?
additional information Mus musculus enzyme is involved in retinoic acids biosynthesis, which are important signaling molecules in embryogenesis and tissue differentiation ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
isozyme type-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme type-1 as fusion protein from Escherichia coli by GST-affinity chromatography, cleavage by thrombin to eliminate the fusion tag Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9-cis-retinal + NAD+ + H2O
-
Mus musculus 9-cis-retinoate + NADH
-
?
all-trans-retinal + NAD+ + H2O
-
Mus musculus all-trans-retinoate + NADH
-
?
all-trans-retinal + NAD+ + H2O preferred substrate of recombinant isozyme type-1 Mus musculus all-trans-retinoate + NADH
-
?
additional information enzyme is involved in retinoic acids biosynthesis, which are important signaling molecules in embryogenesis and tissue differentiation Mus musculus ?
-
?
additional information substrate specificities at different substrate concentrations, no activity with 13-cis-retinal Mus musculus ?
-
?

Subunits

Subunits Comment Organism
? x * 55000, recombinant detagged isozyme type-1, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
RalDH1
-
Mus musculus
retinaldehyde dehydrogenase type-1
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
recombinant isozyme type-1 exhibits 2 pH-optima at pH 7.8 and pH 9.4 with substrates all-trans-retinal and 9-cis-retinal, respectively Mus musculus
9.4
-
recombinant isozyme type-1 exhibits 2 pH-optima at pH 7.8 and pH 9.4 with substrates all-trans-retinal and 9-cis-retinal, respectively Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information kinetics, recombinant isozyme type-1 Mus musculus
0.005
-
Chloral hydrate recombinant isozyme type-1, pH 7.5, 25°C, with substrate all-trans-retinal Mus musculus
0.049
-
acetaldehyde recombinant isozyme type-1, pH 7.5, 25°C, with substrate all-trans-retinal Mus musculus