Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N155A | the mutant shows very low activity compared to the wild type enzyme | Homo sapiens |
N155D | the mutant shows very low activity compared to the wild type enzyme | Homo sapiens |
N155Q | the mutant shows very low activity compared to the wild type enzyme | Homo sapiens |
N169A | inactive | Homo sapiens |
N169D | inactive | Homo sapiens |
N169Q | inactive | Homo sapiens |
R109A | the variant has a similar turnover number but approximately 160fold increased Km as compared with wild type enzyme | Homo sapiens |
R451A | inactive | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00059 | - |
2-aminomuconate 6-semialdehyde | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
0.097 | - |
2-aminomuconate 6-semialdehyde | mutant enzyme R109A, pH and temperature not specified in the publication | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminomuconate 6-semialdehyde + NAD+ + H2O | Homo sapiens | - |
2-aminomuconate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9H2A2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000031 | - |
mutant enzyme N155Q, pH and temperature not specified in the publication | Homo sapiens |
0.0000074 | - |
mutant enzyme N155A, pH and temperature not specified in the publication | Homo sapiens |
0.0000074 | - |
mutant enzyme N155D, pH and temperature not specified in the publication | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminomuconate 6-semialdehyde + NAD+ + H2O | - |
Homo sapiens | 2-aminomuconate + NADH + 2 H+ | - |
? | |
2-hydroxymuconate 6-semialdehyde + NAD+ + H2O | - |
Homo sapiens | 2-hydroxymuconate + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-aminomuconate semialdehyde dehydrogenase | - |
Homo sapiens |
2-AMS dehydrogenase | - |
Homo sapiens |
aldehyde dehydrogenase | - |
Homo sapiens |
ALDH12 | - |
Homo sapiens |
Aldh8a1 | - |
Homo sapiens |
AMSDH | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
2-aminomuconate 6-semialdehyde | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
1.06 | - |
2-aminomuconate 6-semialdehyde | mutant enzyme R109A, pH and temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is the aldehyde dehydrogenase of the kynurenine pathway of tryptophan catabolism | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
2-aminomuconate 6-semialdehyde | mutant enzyme R109A, pH and temperature not specified in the publication | Homo sapiens | |
710 | - |
2-aminomuconate 6-semialdehyde | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens |