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Literature summary for 1.2.1.32 extracted from

  • Reddy, A.M.; Min, K.R.; Lee, K.; Lim, J.Y.; Kim, C.K.; Kim, Y.
    Kinetic property and phylogenic relationship of 2-hydroxymuconic semialdehyde dehydrogenase encoded in tomC gene of Burkholderia cepacia G4 (2004), Arch. Pharm. Res., 27, 570-575.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HB101 Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
2-hydroxy-5-methyl-muconic-6-semialdehyde pH 8.5, 25°C Burkholderia cepacia
0.011
-
2-hydroxymuconate-6-semialdehyde pH 8.5, 25°C Burkholderia cepacia
0.046
-
NAD+ pH 8.5, 25°C, with 2-hydroxy-5-methyl-muconic-6-semialdehyde as substrate Burkholderia cepacia
0.083
-
5-chloro-2-hydroxymuconic-6-semialdehyde pH 8.5, 25°C Burkholderia cepacia
0.294
-
NAD+ pH 8.5, 25°C, with 2-hydroxymuconate-6-semialdehyde as substrate Burkholderia cepacia
1.316
-
NAD+ pH 8.5, 25°C, with 5-chloro-2-hydroxymuconic-6-semialdehyde as substrate Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51740
-
calculated from the predicted amino acid sequence Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O Burkholderia cepacia involved in meta cleavage pathway of aromatic hydrocarbons 4-oxalocrotonate + NADH + H+
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O Burkholderia cepacia G4 involved in meta cleavage pathway of aromatic hydrocarbons 4-oxalocrotonate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia Q83XU8 formerly Pseudomonas cepacia
-
Burkholderia cepacia G4 Q83XU8 formerly Pseudomonas cepacia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-5-methyl-muconic-6-semialdehyde + NAD+ most effective substrate Burkholderia cepacia 2-hydroxy-5-methylmuconate + NADH
-
?
2-hydroxy-5-methyl-muconic-6-semialdehyde + NAD+ most effective substrate Burkholderia cepacia G4 2-hydroxy-5-methylmuconate + NADH
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
-
Burkholderia cepacia 4-oxalocrotonate + NADH + H+
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O involved in meta cleavage pathway of aromatic hydrocarbons Burkholderia cepacia 4-oxalocrotonate + NADH + H+
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
-
Burkholderia cepacia G4 4-oxalocrotonate + NADH + H+
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O involved in meta cleavage pathway of aromatic hydrocarbons Burkholderia cepacia G4 4-oxalocrotonate + NADH + H+
-
?
5-chloro-2-hydroxymuconic-6-semialdehyde + NAD+ poor substrate Burkholderia cepacia 5-chloro-2-hydroxymuconate + NADH
-
?
5-chloro-2-hydroxymuconic-6-semialdehyde + NAD+ poor substrate Burkholderia cepacia G4 5-chloro-2-hydroxymuconate + NADH
-
?
additional information no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Burkholderia cepacia ?
-
?
additional information no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Burkholderia cepacia G4 ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2300
-
5-chloro-2-hydroxymuconic-6-semialdehyde pH 8.5, 25°C Burkholderia cepacia
21900
-
2-hydroxymuconate-6-semialdehyde pH 8.5, 25°C Burkholderia cepacia
99300
-
2-hydroxy-5-methyl-muconic-6-semialdehyde pH 8.5, 25°C Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity in the absence of NAD+ Burkholderia cepacia