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Literature summary for 1.2.1.28 extracted from

  • Tavakoli, A.; Hamzah, A.
    Partial purification and characterization of the recombinant benzaldehyde dehydrogenase from Rhodococcus ruber UKMP-5M (2017), Iran. J. Biotechnol., 15, 67-73 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene xylC, recombinant expression in Escherichia coli strain BL21(DE3) in inclusion bodies. Using higher concentration of lysozyme in lysis buffer, high speed, and long-term centrifugation reduces the protein aggregation Rhodococcus ruber

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2
-
benzaldehyde pH 8.5, 25°C, recombinant enzyme Rhodococcus ruber

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + H2O + NAD+ Rhodococcus ruber
-
benzoate + NADH + 2 H+
-
?
benzaldehyde + H2O + NAD+ Rhodococcus ruber UKMP-5M
-
benzoate + NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus ruber
-
isolated from oilcontaminated soils in Malaysia
-
Rhodococcus ruber UKMP-5M
-
isolated from oilcontaminated soils in Malaysia
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 14fold from Escherichia coli strain BL21(DE3) by anion exchange chromatography Rhodococcus ruber

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
purified recombinant enzyme shows highest activity of 9.4 U/ml Rhodococcus ruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + H2O + NAD+
-
Rhodococcus ruber benzoate + NADH + 2 H+
-
?
benzaldehyde + H2O + NAD+
-
Rhodococcus ruber UKMP-5M benzoate + NADH + 2 H+
-
?
additional information metabolite identification by gas chromatography mass spectrometry (GC-MS) Rhodococcus ruber ?
-
-
additional information metabolite identification by gas chromatography mass spectrometry (GC-MS) Rhodococcus ruber UKMP-5M ?
-
-

Subunits

Subunits Comment Organism
? x * 27000, recombinant enzyme, SDS-PAGE Rhodococcus ruber

Synonyms

Synonyms Comment Organism
BZDH
-
Rhodococcus ruber

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
recombinant enzyme Rhodococcus ruber

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant enzyme, loss of 50% activity Rhodococcus ruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
recombinant enzyme Rhodococcus ruber

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+-dependent enzyme Rhodococcus ruber

General Information

General Information Comment Organism
physiological function enzyme BZDH has the ability to degrade benzaldehyde to less toxic compounds. The BZDH is a critical enzyme for the degradation of aromatic hydrocarbons in Rhodococcus sp. The BZDH from Rhodococcus ruber strain UKMP-5M shows similar function with other aldehyde dehydrogenases Rhodococcus ruber