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Literature summary for 1.2.1.28 extracted from
- Purification and characterization of benzyl alcohol- and benzaldehyde-dehydrogenase from Pseudomonas putida CSV86 (2011), Arch. Microbiol., 193, 553-563.
General Stability
| General Stability | Organism |
|---|---|
| the enzyme loses its activity irreversibly when dialyzed against 50 mM potassium phosphate, pH 6.5, 5% (v/v) glycerol, 2 mM dithiothreitol without benzyl alcohol for 5 h. Enzyme activity is unaffected by varying the concentration of benzyl alcohol | Pseudomonas putida |
| the presence of magnesium may be essential for the stability of the enzyme | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions | Pseudomonas putida |  |
| 2,2'-dipyridyl | 40% residual activity at 1 mM; inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions | Pseudomonas putida | |
| 2-Hydroxybenzaldehyde | substrate inhibition | Pseudomonas putida | |
| 2-Methylbenzaldehyde | substrate inhibition | Pseudomonas putida | |
| 2-naphthaldehyde | substrate inhibition | Pseudomonas putida | |
| 3-Chlorobenzaldehyde | substrate inhibition | Pseudomonas putida | |
| 3-Hydroxybenzaldehyde | substrate inhibition | Pseudomonas putida | |
| 3-Methoxybenzaldehyde | substrate inhibition | Pseudomonas putida | |
| 3-methylbenzaldehyde | substrate inhibition | Pseudomonas putida | |
| 4-Chlorobenzaldehyde | substrate inhibition | Pseudomonas putida | |
| 4-methoxybenzaldehyde | substrate inhibition | Pseudomonas putida | |
| 4-methylbenzaldehyde | substrate inhibition | Pseudomonas putida | |
| benzaldehyde | substrate inhibition; substrate inhibition at higher concentration of benzaldehyde | Pseudomonas putida | |
| additional information | metal chelators (10 mM) like EDTA and EGTA do not affect the activity | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
benzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.0014 | - |
benzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.0018 | - |
3-methylbenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.0018 | - |
3-methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.0028 | - |
3-Hydroxybenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.0028 | - |
3-Chlorobenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.0028 | - |
3-Hydroxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.0028 | - |
3-Chlorobenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.003 | - |
4-Chlorobenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.003 | - |
4-Chlorobenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.0035 | - |
4-methoxybenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.0035 | - |
4-methoxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.0038 | - |
2-naphthaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.0038 | - |
2-naphthaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.02 | - |
4-methylbenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.02 | - |
4-methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.115 | - |
NAD+ | apparent Km-value at substrate saturation, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.129 | - |
3-Methoxybenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.129 | - |
3-Methoxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.377 | - |
2-Methylbenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.377 | - |
2-Methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.497 | - |
2-Hydroxybenzaldehyde | apparent Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.497 | - |
2-Hydroxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5.1 mol Mg2+ per mol enzyme | Pseudomonas putida | |
| Mg2+ | tightly bound | Pseudomonas putida | |
| additional information | BZDH shows the presence of 5.1 mol of magnesium per mole of the enzyme | Pseudomonas putida | |
| additional information | external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, and Zn2+ up to 2.5 mM in the assay mixture do not affect activity | Pseudomonas putida | |
| additional information | external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, or Zn2+ (up to 2.5 mM) does not affect the enzymatic activity | Pseudomonas putida | |
| additional information | metal chelators at 10 mM like EDTA and EGTA do not affect activity | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
subunit molecular mass, determined by SDSPAGE | Pseudomonas putida |
| 117000 | - |
gel filtration | Pseudomonas putida |
| 117000 | - |
native molecular mass of BZDH, determined by Sephacryl S-300-HR gel filtration chromatography | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzaldehyde + NAD+ + H2O | Pseudomonas putida | - |
benzoate + NADH + H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | Pseudomonas putida CSV86 | - |
benzoate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida | - |
MTCC2445 | - |
| Pseudomonas putida CSV86 | - |
- |
- |
| Pseudomonas putida CSV86 | - |
MTCC2445 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE Sephacel column chromatography, Blue agarose column chromatography, Q Sepharose column chromatography, and Sephacryl S-300-HR gel filtration | Pseudomonas putida |
| to apparent homogeneity by ammonium sulfate precipitation and DEAE Sephacel chromatography and resolved by Blue agarose matrix, where BZDH binds to the matrix, BZDH further purified by Sephacryl S-300-HR gel filtration chromatography | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.32 | - |
cell free extract, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida |
| 64 | - |
after 200fold purification, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified enzyme preparations are found to be stable and stored either at 4 or -20°C, stable up to 6 months at -20°C | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-hydroxybenzoate + NADH + H+ | - |
? | |
| 2-hydroxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida CSV86 | 2-hydroxybenzoate + NADH + H+ | - |
? | |
| 2-hydroxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-hydroxybenzoate + NADH + 2 H+ | - |
? | |
| 2-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-methylbenzoate + NADH + 2 H+ | - |
? | |
| 2-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-methylbenzoate + NADH + H+ | - |
? | |
| 2-naphthaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-naphthoate + NADH + H+ | - |
? | |
| 2-naphthaldehyde + NAD+ + H2O | - |
Pseudomonas putida | naphthalene-2-carboxylic acid + NADH + 2 H+ | - |
? | |
| 3-chlorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-chlorobenzoate + NADH + 2 H+ | - |
? | |
| 3-chlorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-chlorobenzoate + NADH + H+ | - |
? | |
| 3-hydroxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-hydroxybenzoate + NADH + H+ | - |
? | |
| 3-hydroxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida CSV86 | 3-hydroxybenzoate + NADH + H+ | - |
? | |
| 3-hydroxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-hydroxybenzoate + NADH + 2 H+ | - |
? | |
| 3-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methoxybenzoate + NADH + H+ | - |
? | |
| 3-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methoxybenzoate + NADH + 2 H+ | - |
? | |
| 3-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methylbenzoate + NADH + H+ | - |
? | |
| 3-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methylbenzoate + NADH + 2 H+ | - |
? | |
| 4-chlorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-chlorobenzoate + NADH + 2 H+ | - |
? | |
| 4-chlorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-chlorobenzoate + NADH + H+ | - |
? | |
| 4-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-methoxybenzoate + NADH + H+ | - |
? | |
| 4-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-methoxybenzoate + NADH + 2 H+ | - |
? | |
| 4-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-methylbenzoate + NADH + H+ | - |
? | |
| 4-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-methylbenzoate + NADH + 2 H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | benzoate + NADH + H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida CSV86 | benzoate + NADH + H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | benzoate + NADH + 2 H+ | - |
ir | |
| additional information | enzyme has a broad substrate specificity accepting mono- and di-aromatic aldehydes but not 2-methoxy derivatives or aliphatic primary aldehydes with 1-6 carbon atoms, no activity with NADP+ or NADPH, BZDH prefers benzaldehydes having substitution either at meta- or para-positions | Pseudomonas putida | ? | - |
? | |
| additional information | the enzyme fails to show activity with NADP+ or NADPH and is inactive with 2-methoxybenzaldehyde, 4-hydroxybenzaldehyde, 2-chlorobenzaldehyde, and 1-naphthaldehyde | Pseudomonas putida | ? | - |
? | |
| additional information | enzyme has a broad substrate specificity accepting mono- and di-aromatic aldehydes but not 2-methoxy derivatives or aliphatic primary aldehydes with 1-6 carbon atoms, no activity with NADP+ or NADPH, BZDH prefers benzaldehydes having substitution either at meta- or para-positions | Pseudomonas putida CSV86 | ? | - |
? | |
| additional information | the enzyme fails to show activity with NADP+ or NADPH and is inactive with 2-methoxybenzaldehyde, 4-hydroxybenzaldehyde, 2-chlorobenzaldehyde, and 1-naphthaldehyde | Pseudomonas putida CSV86 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 57000, SDS-PAGE | Pseudomonas putida |
| homodimer | 2 * 57000 | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| benzaldehyde dehydrogenase | - |
Pseudomonas putida |
| BZDH | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | - |
benzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 14 | - |
benzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 16 | - |
3-Chlorobenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 16 | - |
3-Chlorobenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 23 | - |
3-methylbenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 23 | - |
3-methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 24 | - |
3-Hydroxybenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 24 | - |
3-Hydroxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 33 | - |
2-Hydroxybenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 33 | - |
2-Hydroxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 39 | - |
2-Methylbenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 39 | - |
2-Methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 56 | - |
2-naphthaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 56 | - |
2-naphthaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 67 | - |
4-Chlorobenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 67 | - |
4-Chlorobenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 101 | - |
4-methoxybenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 101 | - |
4-methoxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 120 | - |
4-methylbenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 120 | - |
4-methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 885 | - |
3-Methoxybenzaldehyde | apparent Kcat-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 885 | - |
3-Methoxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
- |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not active with NADP+ | Pseudomonas putida | |
| NAD+ | - |
Pseudomonas putida | |
| NAD+ | dependent on | Pseudomonas putida | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
3-Methoxybenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.003 | - |
4-methylbenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.006 | - |
benzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.006 | - |
benzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 0.008 | - |
2-naphthaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.013 | - |
4-Chlorobenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.035 | - |
4-methoxybenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.051 | - |
3-methylbenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.068 | - |
3-Chlorobenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.131 | - |
3-Hydroxybenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 0.521 | - |
2-Methylbenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 1.808 | - |
2-Hydroxybenzaldehyde | apparent Ki-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the wide substrate specificity of the enzyme enables the strain to detoxify methylnaphthalenes to naphthoic acids efficiently | Pseudomonas putida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60 | - |
2-Hydroxybenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 60 | - |
2-Hydroxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 110 | - |
2-Methylbenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 110 | - |
2-Methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 5700 | - |
3-Chlorobenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 5700 | - |
3-Chlorobenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 6000 | - |
4-methylbenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 6000 | - |
4-methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 6800 | - |
3-Methoxybenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 6800 | - |
3-Methoxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 9000 | - |
3-Hydroxybenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 9000 | - |
3-Hydroxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 10000 | - |
benzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 10000 | - |
benzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 13000 | - |
3-methylbenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 13000 | - |
3-methylbenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 14700 | - |
2-naphthaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 14700 | - |
2-naphthaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 22000 | - |
4-Chlorobenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 22000 | - |
4-Chlorobenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
| 29000 | - |
4-methoxybenzaldehyde | apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication | Pseudomonas putida | |
| 29000 | - |
4-methoxybenzaldehyde | in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication | Pseudomonas putida | |
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