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Literature summary for 1.2.1.24 extracted from

  • Kumar, S.; Kumar, S.; Punekar, N.S.
    Characterization of succinic semialdehyde dehydrogenase from Aspergillus niger (2015), Indian J. Exp. Biol., 53, 67-74 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxybenzaldehyde linear dead-end inhibition, competitive versus succinate semialdehyde, uncompetitive versus NAD+ Aspergillus niger
AMP dead-end inhibition, AMP competes with NAD+ Aspergillus niger
additional information no product inhibition by succinate Aspergillus niger
N-formylglycine
-
Aspergillus niger
NAD+
-
Aspergillus niger
NADH product inhibition, competitive versus NAD+, uncompetitive versus succinate semialdehyde Aspergillus niger
succinate semialdehyde substrate inhibition, uncompetitive versus NAD+ Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and kinetic mechanism, overview Aspergillus niger
0.028
-
succinate semialdehyde pH 9.0 37°C Aspergillus niger
3.1
-
NAD+ pH 9.0 37°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
native enzyme, gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NAD+ + H2O Aspergillus niger
-
succinate + NADH + 2 H+
-
?
succinate semialdehyde + NAD+ + H2O Aspergillus niger NCIM 565
-
succinate + NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger A2R4V5
-
-
Aspergillus niger NCIM 565 A2R4V5
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 9fold from mycelia by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography Aspergillus niger

Reaction

Reaction Comment Organism Reaction ID
succinate semialdehyde + NAD+ + H2O = succinate + NADH + H+ sequential ordered Bi-Bi reaction mechanism Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium grown on gamma-aminobutyric acid Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.277
-
purified enzyme, pH 9.0, 37°C Aspergillus niger

Storage Stability

Storage Stability Organism
-20°C, purified native enzyme, 100 mM diphosphate, pH 8.0, 14 mM 2-mercaptoethanol, 20% glycerol, loss of 50% activity witin 5 days Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NAD+ + H2O
-
Aspergillus niger succinate + NADH + 2 H+
-
?
succinate semialdehyde + NAD+ + H2O the enzyme is specific for the substrates Aspergillus niger succinate + NADH + 2 H+
-
?
succinate semialdehyde + NAD+ + H2O
-
Aspergillus niger NCIM 565 succinate + NADH + 2 H+
-
?
succinate semialdehyde + NAD+ + H2O the enzyme is specific for the substrates Aspergillus niger NCIM 565 succinate + NADH + 2 H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 44000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
SSADH
-
Aspergillus niger
succinic semialdehyde dehydrogenase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Aspergillus niger
NAD+ absolutely specific for Aspergillus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Aspergillus niger
0.44
-
NADH pH 9.0, 37°C, versus succinate semmialdehyde Aspergillus niger
0.55
-
NADH pH 9.0, 37°C, versus NAD+ Aspergillus niger
2.3
-
NAD+ pH 9.0, 37°C Aspergillus niger
3.37
-
4-hydroxybenzaldehyde pH 9.0, 37°C, versus succinate semmialdehyde Aspergillus niger
3.9
-
4-hydroxybenzaldehyde pH 9.0, 37°C, versus NAD+ Aspergillus niger
22.95
-
AMP pH 9.0, 37°C, versus NAD+ Aspergillus niger
31.76
-
AMP pH 9.0, 37°C, versus succinate semmialdehyde Aspergillus niger