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Literature summary for 1.2.1.23 extracted from
- Threonine metabolism in a strain of Bacillus subtilis: enzymes acting on methylglyoxal (1970), Biochim. Biophys. Acta, 222, 668-670.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 60% inhibition at 5 mM, 87% inhibition at 10 mM | Bacillus subtilis |  |
| oxalate | 60% inhibition at 5 mM, 96% inhibition at 10 mM | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
methylglyoxal | - |
Bacillus subtilis | |
| 0.5 | - |
NAD+ | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, 66 mM | Bacillus subtilis | |
| Mg2+ | activation less effective than with Ca2+ | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 500000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methylglyoxal + NAD+ + H2O | Bacillus subtilis | i.e. pyruvaldehyde | pyruvate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.109 | - |
after growth on succinate | Bacillus subtilis |
| 0.14 | - |
after growth on threonine | Bacillus subtilis |
| 1.2 | - |
in crude cell-free extract after growth on threonine | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoaldehydes + NAD+ + H2O | - |
Bacillus subtilis | 2-oxo acids + NADH | - |
? | |
| methylglyoxal + NAD+ + H2O | i.e. pyruvaldehyde | Bacillus subtilis | pyruvate + NADH + H+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.8 | - |
- |
Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus subtilis | |
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Release 2023.1 (January 2023)
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