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Literature summary for 1.2.1.23 extracted from

  • Ray, S.; Ray, M.
    Purification and characterization of NAD and NADP-linked alpha-ketoaldehyde dehydrogenases involved in catalyzing the oxidation of methylglyoxal to pyruvate (1982), J. Biol. Chem., 257, 10566-10570.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol restores the activity after inactivation with p-chloromercuribenzoate Capra hircus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Capra hircus
N-ethylmaleimide 1 mM Capra hircus
p-chloromercuribenzoate
-
Capra hircus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
NAD+
-
Capra hircus
1.7
-
methylglyoxal
-
Capra hircus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration Capra hircus
43000
-
1 * 43000, SDS-PAGE Capra hircus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + NAD+ + H2O Capra hircus i.e. pyruvaldehyde pyruvate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Capra hircus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using acetone powder extract, ammonium sulfate precipitation, calcium phosphate gel treatment, column chromatography on DEAE-cellulose and NAD-Agarose, separated from the NADP-dependent enzyme through affinity chromatography on a thiol-Sepharose column Capra hircus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Capra hircus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.4
-
-
Capra hircus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoaldehydes + NAD+ + H2O
-
Capra hircus 2-oxo acids + NADH
-
?
aldehydes + NAD+ + H2O
-
Capra hircus carboxylic acids + NADH
-
?
methylglyoxal + NAD+ + H2O i.e. pyruvaldehyde Capra hircus pyruvate + NADH + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Capra hircus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Capra hircus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Capra hircus