Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | restores the activity after inactivation with p-chloromercuribenzoate | Capra hircus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Capra hircus | |
N-ethylmaleimide | 1 mM | Capra hircus | |
p-chloromercuribenzoate | - |
Capra hircus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
NAD+ | - |
Capra hircus | |
1.7 | - |
methylglyoxal | - |
Capra hircus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Capra hircus |
43000 | - |
1 * 43000, SDS-PAGE | Capra hircus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NAD+ + H2O | Capra hircus | i.e. pyruvaldehyde | pyruvate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capra hircus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using acetone powder extract, ammonium sulfate precipitation, calcium phosphate gel treatment, column chromatography on DEAE-cellulose and NAD-Agarose, separated from the NADP-dependent enzyme through affinity chromatography on a thiol-Sepharose column | Capra hircus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Capra hircus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.4 | - |
- |
Capra hircus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoaldehydes + NAD+ + H2O | - |
Capra hircus | 2-oxo acids + NADH | - |
? | |
aldehydes + NAD+ + H2O | - |
Capra hircus | carboxylic acids + NADH | - |
? | |
methylglyoxal + NAD+ + H2O | i.e. pyruvaldehyde | Capra hircus | pyruvate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Capra hircus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Capra hircus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Capra hircus |