Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.23 extracted from

  • Rhee, H.; Watanabe, K.; Murata, K.; Kimura, A.
    Metabolism of 2-ketoaldehydes in bacteria: oxidative conversion of methylglyoxal to pyruvate by an enzyme from Pseudomonas putida (1987), Agric. Biol. Chem., 51, 1059-1066.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation Pseudomonas putida
fructose 1,6-bisphosphate slightly activation Pseudomonas putida
glutathione activation Pseudomonas putida
Glycerate 3-phosphate slightly activation Pseudomonas putida
L-cysteine activation Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 30-40% inhibition at 1.0 mM Pseudomonas putida
Cu2+ 30-40% inhibition at 1.0 mM Pseudomonas putida
Hg2+ almost complete inhibition at 1 mM HgCl2 Pseudomonas putida
Mn2+ 30-40% inhibition at 1.0 mM Pseudomonas putida
N-ethylmaleimide 1 mM Pseudomonas putida
NADP+ 50% inhibition at 2.0 mM and 80% at 5.0 mM Pseudomonas putida
Ni2+ 30-40% inhibition at 1.0 mM Pseudomonas putida
p-chloromercuribenzoate 0.1 mM Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NAD+
-
Pseudomonas putida
1
-
methylglyoxal
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 35% activation at 2.5 mM and 50% activation at 5.0 mM MgCl2 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
1 * 42000, SDS-PAGE Pseudomonas putida
43000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + NAD+ + H2O Pseudomonas putida i.e. pyruvaldehyde pyruvate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography on DEAE-cellulose, Butyl-Toyopearl 650M, Sephadex G-150, hydroxylapatite, Blue-dextran and Sepharose CL-6B Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
4°C or -20°C, 10 mM potassium phosphate buffer, pH 8.0, 3 weeks Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoaldehydes + NAD+ + H2O
-
Pseudomonas putida 2-oxo acids + NADH
-
?
aldehydes + NAD+ + H2O
-
Pseudomonas putida carboxylic acids + NADH
-
?
methylglyoxal + NAD+ + H2O i.e. pyruvaldehyde Pseudomonas putida pyruvate + NADH + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida
NADP+ can not substitute for NAD Pseudomonas putida