Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activation | Pseudomonas putida | |
fructose 1,6-bisphosphate | slightly activation | Pseudomonas putida | |
glutathione | activation | Pseudomonas putida | |
Glycerate 3-phosphate | slightly activation | Pseudomonas putida | |
L-cysteine | activation | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 30-40% inhibition at 1.0 mM | Pseudomonas putida | |
Cu2+ | 30-40% inhibition at 1.0 mM | Pseudomonas putida | |
Hg2+ | almost complete inhibition at 1 mM HgCl2 | Pseudomonas putida | |
Mn2+ | 30-40% inhibition at 1.0 mM | Pseudomonas putida | |
N-ethylmaleimide | 1 mM | Pseudomonas putida | |
NADP+ | 50% inhibition at 2.0 mM and 80% at 5.0 mM | Pseudomonas putida | |
Ni2+ | 30-40% inhibition at 1.0 mM | Pseudomonas putida | |
p-chloromercuribenzoate | 0.1 mM | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | - |
Pseudomonas putida | |
1 | - |
methylglyoxal | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 35% activation at 2.5 mM and 50% activation at 5.0 mM MgCl2 | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
1 * 42000, SDS-PAGE | Pseudomonas putida |
43000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NAD+ + H2O | Pseudomonas putida | i.e. pyruvaldehyde | pyruvate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using column chromatography on DEAE-cellulose, Butyl-Toyopearl 650M, Sephadex G-150, hydroxylapatite, Blue-dextran and Sepharose CL-6B | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C or -20°C, 10 mM potassium phosphate buffer, pH 8.0, 3 weeks | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoaldehydes + NAD+ + H2O | - |
Pseudomonas putida | 2-oxo acids + NADH | - |
? | |
aldehydes + NAD+ + H2O | - |
Pseudomonas putida | carboxylic acids + NADH | - |
? | |
methylglyoxal + NAD+ + H2O | i.e. pyruvaldehyde | Pseudomonas putida | pyruvate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NADP+ | can not substitute for NAD | Pseudomonas putida |