Literature summary for 1.2.1.19 extracted from

Kanwal, S.; Incharoensakdi, A.
GABA synthesis mediated by gamma-aminobutanal dehydrogenase in Synechocystis sp. PCC6803 with disrupted glutamate and alpha-ketoglutarate decarboxylase genes (2020), Plant Sci., 290, 110287 .

Search for more literature for 1.2.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
gene gadbh, quantitative RT-PCR enzyme expression analysis	Synechocystis sp. PCC 6803

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a series of Synechocystis sp. PCC6803 mutants lacking enzymes of TCAC shunt, GABA synthesis and polyamine degradation pathway, i.e. DELTAkgd, DELTAgad:DELTAkgd and DELTAgabdh encoding glutamate and 2-oxoglutarate decarboxylases, respectively. Subcloning in Escherichia coli strain DH5alpha. The level of chl a content is significantly decreased in both DELTAgabdh and DELTAgad:DELTAkgd strains as compared to that in the wild-type. Similarly, lower O2 evolution rate is observed in both mutants signifying a decline in photosynthetic efficiency, phenotype, overview. The mutant strain with disrupted gene gabdh shows an increase in gamma-aminobutyric acid (GABA), glutamate, succinate, and spermidine levels, while the DELTAgad:DELTAkgd strain shows upregulated expression of gene gadbh	Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-aminobutyraldehyde + NAD+ + H2O	Synechocystis sp. PCC 6803	-	4-aminobutanoate + NADH + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp. PCC 6803	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-aminobutyraldehyde + NAD+ + H2O	-	Synechocystis sp. PCC 6803	4-aminobutanoate + NADH + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
gadbh	-	Synechocystis sp. PCC 6803
gamma-aminobutanal dehydrogenase	-	Synechocystis sp. PCC 6803

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Synechocystis sp. PCC 6803

General Information

General Information	Comment	Organism
malfunction	a mutant strain with disrupted gene gabdh shows an increase in gamma-aminobutyric acid (GABA), glutamate, succinate, and spermidine levels demonstrating a link between spermidine degradation and GABA synthesis in cyanobacteria. A DELTAgad:DELTAkgd strain shows upregulated expression of gene gadbh. Transcription levels of genes related to GABA metabolism in WT and mutants, overview	Synechocystis sp. PCC 6803
physiological function	role of gamma-aminobutanal dehydrogenase in maintaining an intact tricarboxylic acid cycle in Synechocystis. The mutational analysis suggests a possible existence of the Spd to gamma-AB to GABA pathway in Synechocystis	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)