Cloned (Comment) | Organism |
---|---|
gene ataldh10A8, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana protoplast, recombinant expression of His-tagged enzyme in in Escherichia coli | Arabidopsis thaliana |
gene ataldh10A9, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana protoplast, recombinant expression of His-tagged enzyme in in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a ataldh10A8 T-DNA-insertion mutant that has lower levels of GABA than wild-type plants in response to salinity treatment and are more prone to salinity stress | Arabidopsis thaliana |
additional information | generation of a ataldh10A9 T-DNA-insertion mutant that has lower levels of GABA than wild-type plants in response to salinity treatment and are more prone to salinity stress | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Arabidopsis thaliana | |
0.0132 | - |
NAD+ | with 4-aminobutanal, pH 8.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
0.0144 | - |
4-aminobutanal | pH 8.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
0.0173 | - |
4-aminobutanal | pH 9.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
0.0208 | - |
NAD+ | with 3-aminopropanal, pH 8.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
0.0251 | - |
3-aminopropanal | pH 8.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
0.0736 | - |
NAD+ | with 3-aminopropanal, pH 9.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
0.086 | - |
NAD+ | with 4-aminobutanal, pH 9.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
0.46 | - |
3-aminopropanal | pH 9.5, 22°C, recombinant enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | AtALDH10A8 is cytosolic, although the N-terminal 140 amino acid sequence of AtALDH10A8 localizes to the plastid and to the leucoplast | Arabidopsis thaliana | 9507 | - |
cytosol | AtALDH10A8 is cytosolic, although the N-terminal 140 amino acid sequence of AtALDH10A8 localizes to the plastid and to the leucoplast | Arabidopsis thaliana | 5829 | - |
peroxisome | - |
Arabidopsis thaliana | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminobutanal + NAD+ + H2O | Arabidopsis thaliana | best substrate | 3-aminobutanoate + NADH + 2 H+ | - |
? | |
3-aminopropanal + NAD+ + H2O | Arabidopsis thaliana | best substrate | 3-aminopropanoate + NADH + H+ | - |
? | |
4-aminobutanal + NAD+ + H2O | Arabidopsis thaliana | - |
4-aminobutanoate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S795 | - |
- |
Arabidopsis thaliana | Q9STS1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from in Escherichia coli by affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminobutanal + NAD+ + H2O | best substrate | Arabidopsis thaliana | 3-aminobutanoate + NADH + 2 H+ | - |
? | |
3-aminopropanal + NAD+ + H2O | best substrate | Arabidopsis thaliana | 3-aminopropanoate + NADH + H+ | - |
? | |
4-aminobutanal + NAD+ + H2O | - |
Arabidopsis thaliana | 4-aminobutanoate + NADH + 2 H+ | - |
? | |
additional information | maximal activity and catalytic efficiency are obtained with NAD+ and 3-aminopropanal, followed by 4-aminobutanal, negligible activity is obtained with betaine aldehyde. The betaine-aldehyde dehydrogenase ADH10A8 acts as 4-aminobutanal dehydrogenase | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALDH10A8 | - |
Arabidopsis thaliana |
ALDH10A9 | - |
Arabidopsis thaliana |
AMADH | - |
Arabidopsis thaliana |
More | cf. EC 1.2.1.8 | Arabidopsis thaliana |
NAD+-dependent aminoaldehyde dehydrogenase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Arabidopsis thaliana |
10.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | the enzyme retains approximately 20% of its maximal substrate-limited, 3-aminopropanal-dependent activity at pH 7.5 and is inactive at pH 6.5 | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | AtALDH109 gene is expressed constitutively in Arabidopsis thaliana, although it has been reported to be weakly induced by salinity and dehydration | up |
Arabidopsis thaliana | AtALDH10A8 gene is expressed constitutively in Arabidopsis thaliana, although it has been reported to be weakly induced by salinity and dehydration | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the aldehyde dehydrogenase 10 family | Arabidopsis thaliana |
malfunction | root growth of single loss-of-function mutants is more sensitive to salinity than wild-type plants, and this is accompanied by reduced GABA accumulation | Arabidopsis thaliana |
physiological function | the enzyme shows maximal activity and catalytic efficiency with NAD+ and 3-aminopropanal, followed by 4-aminobutanal. Negligible activity is obtained with betaine aldehyde. NAD+ reduction is accompanied by the production of gamma-aminobutyrate, GABA, and beta-alanine, respectively, with 4-aminobutanal and 3-aminopropanal as substrates | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
3-aminopropanal | pH 9.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
40 | - |
NAD+ | with 3-aminopropanal, pH 8.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
60 | - |
3-aminopropanal | pH 8.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
60 | - |
NAD+ | with 3-aminopropanal, pH 9.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
150 | - |
NAD+ | with 4-aminobutanal, pH 9.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
260 | - |
NAD+ | with 4-aminobutanal, pH 8.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
820 | - |
4-aminobutanal | pH 9.5, 22°C, recombinant enzyme | Arabidopsis thaliana | |
1030 | - |
4-aminobutanal | pH 8.5, 22°C, recombinant enzyme | Arabidopsis thaliana |