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Literature summary for 1.2.1.16 extracted from

  • Corregido, M.; Asencion Diez, M.; Iglesias, A.; Piattoni, C.
    New pieces to the carbon metabolism puzzle of Nitrosomonas europaea kinetic characterization of glyceraldehyde-3 phosphate and succinate semialdehyde dehydrogenases (2019), Biochimie, 158, 238-245 .
    View publication on PubMed
Search for more literature for 1.2.1.16

Cloned(Commentary)

Cloned (Comment) Organism
gene gapD, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Nitrosomonas europaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 succinate semialdehyde pH 8.5, 30°C, recombinant enzyme, with NADP+ Nitrosomonas europaea
0.004
-
 NADP+ pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde Nitrosomonas europaea
0.007
-
 succinate semialdehyde pH 8.5, 30°C, recombinant enzyme, with NAD+ Nitrosomonas europaea
1.12
-
 NAD+ pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde Nitrosomonas europaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NAD+ + H2O Nitrosomonas europaea
-
 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O Nitrosomonas europaea NBRC 14298
-
 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O Nitrosomonas europaea ATCC 19718
-
 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O Nitrosomonas europaea KCTC 2705
-
 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O Nitrosomonas europaea CIP 103999
-
 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O Nitrosomonas europaea
-
 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O Nitrosomonas europaea NBRC 14298
-
 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O Nitrosomonas europaea ATCC 19718
-
 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O Nitrosomonas europaea KCTC 2705
-
 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O Nitrosomonas europaea CIP 103999
-
 succinate + NADPH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea Q82TA7
-
-
Nitrosomonas europaea ATCC 19718 Q82TA7
-
-
Nitrosomonas europaea CIP 103999 Q82TA7
-
-
Nitrosomonas europaea KCTC 2705 Q82TA7
-
-
Nitrosomonas europaea NBRC 14298 Q82TA7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Nitrosomonas europaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is also active with D-glyceraldehyde 3-phosphate (Ga3P), cf. EC 1.2.1.90. Values obtained reflect a 3500 and 87fold higher catalytic efficiency when SSA/NADP+ or SSA/NAD+ pairs, respectively, are used instead of Ga3P/NADP+ or Ga3P/NAD+. Preference for NADP+ when SSA is the substrate Nitrosomonas europaea ?
-
-
additional information the enzyme is also active with D-glyceraldehyde 3-phosphate (Ga3P), cf. EC 1.2.1.90. Values obtained reflect a 3500 and 87fold higher catalytic efficiency when SSA/NADP+ or SSA/NAD+ pairs, respectively, are used instead of Ga3P/NADP+ or Ga3P/NAD+. Preference for NADP+ when SSA is the substrate Nitrosomonas europaea NBRC 14298 ?
-
-
additional information the enzyme is also active with D-glyceraldehyde 3-phosphate (Ga3P), cf. EC 1.2.1.90. Values obtained reflect a 3500 and 87fold higher catalytic efficiency when SSA/NADP+ or SSA/NAD+ pairs, respectively, are used instead of Ga3P/NADP+ or Ga3P/NAD+. Preference for NADP+ when SSA is the substrate Nitrosomonas europaea ATCC 19718 ?
-
-
additional information the enzyme is also active with D-glyceraldehyde 3-phosphate (Ga3P), cf. EC 1.2.1.90. Values obtained reflect a 3500 and 87fold higher catalytic efficiency when SSA/NADP+ or SSA/NAD+ pairs, respectively, are used instead of Ga3P/NADP+ or Ga3P/NAD+. Preference for NADP+ when SSA is the substrate Nitrosomonas europaea KCTC 2705 ?
-
-
additional information the enzyme is also active with D-glyceraldehyde 3-phosphate (Ga3P), cf. EC 1.2.1.90. Values obtained reflect a 3500 and 87fold higher catalytic efficiency when SSA/NADP+ or SSA/NAD+ pairs, respectively, are used instead of Ga3P/NADP+ or Ga3P/NAD+. Preference for NADP+ when SSA is the substrate Nitrosomonas europaea CIP 103999 ?
-
-
succinate semialdehyde + NAD+ + H2O
-
 Nitrosomonas europaea succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Nitrosomonas europaea NBRC 14298 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Nitrosomonas europaea ATCC 19718 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Nitrosomonas europaea KCTC 2705 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Nitrosomonas europaea CIP 103999 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Nitrosomonas europaea succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O NADP+ is the preferred cofactor Nitrosomonas europaea succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Nitrosomonas europaea NBRC 14298 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O NADP+ is the preferred cofactor Nitrosomonas europaea NBRC 14298 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Nitrosomonas europaea ATCC 19718 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O NADP+ is the preferred cofactor Nitrosomonas europaea ATCC 19718 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Nitrosomonas europaea KCTC 2705 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O NADP+ is the preferred cofactor Nitrosomonas europaea KCTC 2705 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Nitrosomonas europaea CIP 103999 succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O NADP+ is the preferred cofactor Nitrosomonas europaea CIP 103999 succinate + NADPH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
More see also EC 1.2.1.90 Nitrosomonas europaea
SSA dehydrogenase
-
 Nitrosomonas europaea
SSADHase
-
 Nitrosomonas europaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Nitrosomonas europaea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 NAD+ pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde Nitrosomonas europaea
0.483
-
 succinate semialdehyde pH 8.5, 30°C, recombinant enzyme, with NAD+ Nitrosomonas europaea
0.583
-
 succinate semialdehyde pH 8.5, 30°C, recombinant enzyme, with NADP+ Nitrosomonas europaea
0.617
-
 NADP+ pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde Nitrosomonas europaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Nitrosomonas europaea

Cofactor

Cofactor Comment Organism Structure
additional information the Km value for NADP+ is 26fold higher than that for NAD+. The Km value for SSA increases 3fold with NADP+ in comparison with NAD+ Nitrosomonas europaea
NAD+
-
 Nitrosomonas europaea
NADP+ preferred cofactor Nitrosomonas europaea

General Information

General Information Comment Organism
physiological function enzyme GabD irreversibly oxidizes D-glyceraldehyde 3-phosphate (Ga3P) to 3-phospho-D-glycerate (3Pglycerate) using NAD+ or NADP+, thus resembling a non-phosphorylating Ga3PDHase (EC 1.2.1.90). But the enzyme shows about 6fold higher Km value and three orders of magnitude higher catalytic efficiency with succinate semialdehyde (SSA) and NADP+. Indeed, the GabD protein identity corresponds to an SSA dehydrogenase (SSADHase). As an SSADHase, GabD physiologically acts producing succinate and preferentially NADPH over NADH, thus being part of an alternative pathway of the tricarboxylic acid cycle converting alpha-ketoglutarate to succinate Nitrosomonas europaea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.4
-
 NAD+ pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde Nitrosomonas europaea
70.4
-
 succinate semialdehyde pH 8.5, 30°C, recombinant enzyme, with NAD+ Nitrosomonas europaea
154.3
-
 NADP+ pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde Nitrosomonas europaea
194.3
-
 succinate semialdehyde pH 8.5, 30°C, recombinant enzyme, with NADP+ Nitrosomonas europaea