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Literature summary for 1.2.1.16 extracted from

  • de Carvalho, L.P.; Ling, Y.; Shen, C.; Warren, J.D.; Rhee, K.Y.
    On the chemical mechanism of succinic semialdehyde dehydrogenase (GabD1) from Mycobacterium tuberculosis (2011), Arch. Biochem. Biophys., 509, 90-99.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is not influenced by 5 mM EDTA Mycobacterium tuberculosis
succinate semialdehyde
-
Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
succinate semialdehyde with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
0.0092
-
NAD+ in the presence of 10 mM Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
0.0133
-
succinate semialdehyde with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
0.0613
-
NADP+ in the absence of Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Mycobacterium tuberculosis
Co2+
-
Mycobacterium tuberculosis
Mg2+ 100% activity at 10 mM Mg2+. With Mg2+ there is a 4fold decrease in the Km value for succinate semialdehyde and a 6fold decrease in the Km value for NADP+ Mycobacterium tuberculosis
Mn2+
-
Mycobacterium tuberculosis
additional information not activated by Cd2+ and Zn2+ Mycobacterium tuberculosis
Ni2+
-
Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycobacterium tuberculosis other aldehydes, such as formaldehyde, acetaldehyde and glutaraldehyde, are very poor substrates ?
-
?
succinate semialdehyde + NAD+ + H2O Mycobacterium tuberculosis
-
succinate + NADH + H+
-
?
succinate semialdehyde + NADP+ + H2O Mycobacterium tuberculosis
-
succinate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Oxidation Stability

Oxidation Stability Organism
there is no significant loss of catalytic activity, after up to 40 min of incubation of isoform GabD1 with 1 mM H2O2 at pH 7.4 Mycobacterium tuberculosis

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information other aldehydes, such as formaldehyde, acetaldehyde and glutaraldehyde, are very poor substrates Mycobacterium tuberculosis ?
-
?
succinate semialdehyde + NAD+ + H2O
-
Mycobacterium tuberculosis succinate + NADH + H+
-
?
succinate semialdehyde + NADP+ + H2O
-
Mycobacterium tuberculosis succinate + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
GabD1 isoform Mycobacterium tuberculosis
succinic semialdehyde dehydrogenase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
NADP+ in the absence of Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
1.9
-
succinate semialdehyde with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.7
-
NAD+ in the presence of 10 mM Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
4.7
-
succinate semialdehyde with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 the enzyme cannot be assayed below pH 5.5, due to precipitation and loss of activity Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NAD+ long incubations lead to a modest utilization of NAD+ as substrate Mycobacterium tuberculosis
NADP+ although long incubations lead to a modest utilization of NAD+ as substrate, NADP+ is the preferred substrate Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0628
-
succinate semialdehyde in the absence of Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
140
-
succinate semialdehyde with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
210
-
NADP+ in the absence of Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
290
-
NAD+ in the presence of 10 mM Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis
1600
-
succinate semialdehyde with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C Mycobacterium tuberculosis