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Literature summary for 1.2.1.16 extracted from

  • Esser, D.; Kouril, T.; Talfournier, F.; Polkowska, J.; Schrader, T.; Bräsen, C.; Siebers, B.
    Unraveling the function of paralogs of the aldehyde dehydrogenase super family from Sulfolobus solfataricus (2013), Extremophiles, 17, 205-216.
    View publication on PubMed
Search for more literature for 1.2.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3)-RIL cells Saccharolobus solfataricus
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 Succinic semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
0.004
-
 Succinic semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
0.01
-
 NADP+ at pH 6.5 and 70°C Saccharolobus solfataricus
0.03
-
 Succinic semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
0.1
-
 2-oxoglutarate semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
0.2
-
 2-oxoglutarate semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
0.3
-
 2-oxoglutarate semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
9.1
-
 NAD+ in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
11.1
-
 NAD+ at pH 6.5 and 70°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51600
-
-
 Saccharolobus solfataricus
51600
-
 2 * 51600, SDS-PAGE Escherichia coli
114000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate semialdehyde + NAD+ + H2O Escherichia coli
-
 2-oxoglutarate + NADH + H+
-
 ?
2-oxoglutarate semialdehyde + NADP+ + H2O Escherichia coli
-
 2-oxoglutarate + NADPH + H+
-
 ?
acetaldehyde + NADP+ + H2O Escherichia coli
-
 succinate + NADPH + H+
-
 ?
additional information Escherichia coli almost no activity with DL-glyceraldehyde, DL-glyceraldehyde 3-phosphate, and glycoaldehyde ?
-
 ?
succinic semialdehyde + NAD+ + H2O Escherichia coli
-
 succinate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
UNO Q-12 column chromatography Escherichia coli
UNO Q-12 column chromatography and Superdex 200 gel filtration Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate semialdehyde + NAD+ + H2O
-
 Escherichia coli 2-oxoglutarate + NADH + H+
-
 ?
2-oxoglutarate semialdehyde + NAD+ + H2O
-
 Saccharolobus solfataricus 2-oxoglutarate + NADH + H+
-
 ?
2-oxoglutarate semialdehyde + NADP+ + H2O
-
 Escherichia coli 2-oxoglutarate + NADPH + H+
-
 ?
2-oxoglutarate semialdehyde + NADP+ + H2O
-
 Saccharolobus solfataricus 2-oxoglutarate + NADPH + H+
-
 ?
acetaldehyde + NADP+ + H2O
-
 Escherichia coli succinate + NADPH + H+
-
 ?
additional information almost no activity with DL-glyceraldehyde, DL-glyceraldehyde 3-phosphate, and glycoaldehyde Escherichia coli ?
-
 ?
additional information almost no activity with D,L-glyceraldehyde, D,L-glyceraldehyde-3-phosphate, and D,L-glycolaldehyde Saccharolobus solfataricus ?
-
 ?
succinic semialdehyde + NAD+ + H2O
-
 Escherichia coli succinate + NADH + H+
-
 ?
succinic semialdehyde + NAD+ + H2O
-
 Saccharolobus solfataricus succinate + NADH + H+
-
 ?
succinic semialdehyde + NADP+ + H2O
-
 Saccharolobus solfataricus succinate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 51600, SDS-PAGE Saccharolobus solfataricus
homodimer 2 * 51600, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
ALDH
-
 Saccharolobus solfataricus
gapN-2
-
 Escherichia coli
gapN-2
-
 Saccharolobus solfataricus
SSADH-II
-
 Escherichia coli
SSADH-II
-
 Saccharolobus solfataricus
SSO1842
-
 Escherichia coli
SSO1842
-
 Saccharolobus solfataricus
succinic semialdehyde dehydrogenase
-
 Saccharolobus solfataricus
succinic semialdehyde:NAD(P)+ oxidoreductase
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1
-
 2-oxoglutarate semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
16.5
-
 NADP+ at pH 6.5 and 70°C Saccharolobus solfataricus
16.8
-
 Succinic semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
18.4
-
 Succinic semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
21.2
-
 2-oxoglutarate semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
22.6
-
 Succinic semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
27.3
-
 2-oxoglutarate semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
69
-
 NAD+ in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
136.3
-
 NAD+ at pH 6.5 and 70°C Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli
NAD+
-
 Saccharolobus solfataricus
NADP+
-
 Saccharolobus solfataricus
NADP+ preferred cofactor Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.3
-
 NAD+ in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
12.3
-
 NAD+ at pH 6.5 and 70°C Saccharolobus solfataricus
134.1
-
 2-oxoglutarate semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
134.1
-
 2-oxoglutarate semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
215.3
-
 2-oxoglutarate semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1789
-
 Succinic semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
3066
-
 NADP+ in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
3454
-
 Succinic semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
3454
-
 Succinic semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
30660
-
 NADP+ at pH 6.5 and 70°C Saccharolobus solfataricus