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Literature summary for 1.2.1.15 extracted from
- Studies on malonic semialdehyde dehydrogenase from Pseudomonas aeruginosa (1961), Biochim. Biophys. Acta, 50, 147-152.
General Stability
| General Stability | Organism |
|---|---|
| 1 mM mercaptoethanol is essential for stability | Pseudomonas aeruginosa |
| unstable in dilute solution | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Pseudomonas aeruginosa |  |
| Cu2+ | - |
Pseudomonas aeruginosa | |
| glyoxylate | competitive inhibitor | Pseudomonas aeruginosa | |
| HgCl2 | - |
Pseudomonas aeruginosa | |
| malonate | competitive inhibitor | Pseudomonas aeruginosa | |
| Ni2+ | - |
Pseudomonas aeruginosa | |
| p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
| phosphate | - |
Pseudomonas aeruginosa | |
| succinate semialdehyde | non-competitive inhibitor | Pseudomonas aeruginosa | |
| Zn2+ | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
malonate semialdehyde | - |
Pseudomonas aeruginosa | |
| 0.11 | - |
NAD+ | - |
Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | increases activity 42% at 1 mM | Pseudomonas aeruginosa | |
| Mg2+ | increases activity 64% at 1 mM | Pseudomonas aeruginosa | |
| Mn2+ | increases activity 27% at 1 mM | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-oxopropanoate + NAD+ + H2O | Pseudomonas aeruginosa | - |
malonate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.203 | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, 1 month | Pseudomonas aeruginosa |
| 0°C, bovine serum albumin, NAD+, 1 week | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-oxopropanoate + NAD+ + H2O | - |
Pseudomonas aeruginosa | malonate + NADH | - |
? | |
| additional information | no activity with formaldehyde, acetaldehyde, propionaldehyde, isovaleraldehyde, benzaldehyde, anisaldehyde, glyceraldehyde, glyceraldehyde-3-phosphate, glyoxylate, succinic semialdehyde | Pseudomonas aeruginosa | ? | - |
? | |
| NAD(P)+ + 3-oxopropanoate + H2O | - |
Pseudomonas aeruginosa | malonate + NAD(P)H | - |
ir | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
with NAD+ and bovine serum albumin at pH 8.7 | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
malonate semialdehyde + NAD+ | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas aeruginosa | |
| NADP+ | less than 10% activity compared with NAD+ | Pseudomonas aeruginosa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
succinate semialdehyde | - |
Pseudomonas aeruginosa | |
| 0.57 | - |
malonate | - |
Pseudomonas aeruginosa | |
| 1.8 | - |
glyoxylate | - |
Pseudomonas aeruginosa | |
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