KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0734 | - |
D-Glyceraldehyde-3-phosphate | in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C | Sardina pilchardus | |
0.092 | - |
NAD+ | in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C | Sardina pilchardus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sardina pilchardus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
4 * 37000, denaturing SDS-PAGE | Sardina pilchardus |
38500 | - |
4 * 38500, non-denaturing PAGE | Sardina pilchardus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sardina pilchardus | - |
European pilchard | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation and Blue Sepharose column chromatography | Sardina pilchardus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Sardina pilchardus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.46 | - |
crude extract | Sardina pilchardus |
35.75 | - |
after 77.71fold purification | Sardina pilchardus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Sardina pilchardus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 37000, denaturing SDS-PAGE | Sardina pilchardus |
homotetramer | 4 * 38500, non-denaturing PAGE | Sardina pilchardus |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Sardina pilchardus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Sardina pilchardus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 32 | - |
Sardina pilchardus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 60 | pre-incubation for 10 min at temperatures varying between 15°C and 30°C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35°C and results in total activity loss at 60°C | Sardina pilchardus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sardina pilchardus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Sardina pilchardus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent | Sardina pilchardus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sardina pilchardus | isoelectric focusing | - |
7.9 |