Protein Variants | Comment | Organism |
---|---|---|
D282G | enzyme exists as dimer and tetramer, the tetramer is inactive, the dimer is slightly active, 650fold decrease in turnover number for NAD+, 5.8fold increase in Km-value for NAD+ compared to wild-type enzyme | Geobacillus stearothermophilus |
N313T | mutant enzyme with a drastic decrease in thermostability, weakening of cooperative interactions between the catalytic and the cofactor domains and an inefficient binding of NAD+, mutant enzyme exists only as tetramer, 65fold decrease in turnover number for NAD+, 50fold increase in Km-value for NAD+ compared to wild-type enzyme | Geobacillus stearothermophilus |
T34Q/T39S/L43Q | drastic decrease in thermostability, inefficient NAD+ binding, enzyme exists as dimer and tetramer, the tetramer is inactive, the dimer is slightly active, 650fold decrease in turnover number for NAD+, 4fold increase in Km-value for NAD+ compared to wild-type enzyme | Geobacillus stearothermophilus |
W310F | mutant enzyme with a drastic decrease in thermostability, mutant enzyme exists only as tetramer, 2fold increase of Km-value for NAD+, 1.3fold decrease in turnover number compared to wild-type enzyme | Geobacillus stearothermophilus |
Y283V | mutant enzyme with a drastic decrease in thermostability, dimeric form is inactive, KM-value and turnover-number of tetramer are nearly identical to that of the wild-type | Geobacillus stearothermophilus |
Y46G/R52G | inactive mutant enzyme, only exists as dimer | Geobacillus stearothermophilus |
Y46G/S48G | inactive mutant enzyme, only exists as dimer | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, wild-type | Geobacillus stearothermophilus | |
0.06 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant Y283V | Geobacillus stearothermophilus | |
0.1 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant W310F | Geobacillus stearothermophilus | |
0.2 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant D282G | Geobacillus stearothermophilus | |
0.29 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant T34Q/T39S/L43Q | Geobacillus stearothermophilus | |
2.5 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant N313T | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Geobacillus stearothermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
62 | - |
maximum of thermal transition peak of tetrameric form of mutant apoenzyme N313T | Geobacillus stearothermophilus |
64.8 | - |
maximum of thermal transition peak of dimeric mutant apoenzyme T34Q/T39S/L43Q | Geobacillus stearothermophilus |
64.8 | - |
maximum of thermal transition peak of tetrameric mutant apoenzyme T34Q/T39S/L43Q | Geobacillus stearothermophilus |
65.4 | - |
maximum of thermal transition peak of dimeric mutant apoenzyme Y46G/S48G | Geobacillus stearothermophilus |
65.5 | - |
maximum of thermal transition peak of dimeric mutant apoenzyme Y46G/R52G | Geobacillus stearothermophilus |
68.4 | - |
maximum of thermal transition peak of tetrameric mutant apoenzyme Y283V | Geobacillus stearothermophilus |
70.3 | - |
maximum of thermal transition peak of tetrameric mutant apoenzyme D283G | Geobacillus stearothermophilus |
70.7 | - |
maximum of thermal transition peak of tetrameric mutant apoenzyme W310F | Geobacillus stearothermophilus |
71 | - |
maximum of thermal transition peak of dimeric mutant apoenzyme D282G | Geobacillus stearothermophilus |
73.5 | - |
maximum of thermal transition peak of dimeric mutant apoenzyme Y283V | Geobacillus stearothermophilus |
78.5 | - |
maximum of thermal transition peak of tetrameric form of wild-type apoenzyme | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant D282G | Geobacillus stearothermophilus | |
0.1 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant T34Q/T39S/L43Q | Geobacillus stearothermophilus | |
1 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant N313T | Geobacillus stearothermophilus | |
51 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant W310F | Geobacillus stearothermophilus | |
65 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, wild-type | Geobacillus stearothermophilus | |
73 | - |
NAD+ | pH 8.0, 25°C, tetrameric enzyme form, mutant Y283V | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus |