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Literature summary for 1.2.1.102 extracted from

  • Lee, Y.; Nelson, M.; Snell, E.
    Enzymes of vitamin B6 degradation. Purification and properties of isopyridoxal dehydrogenase and 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid dehydrogenase (1986), J. Biol. Chem., 261, 15106-15111 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-deoxyisopyridoxal competitive Arthrobacter sp. Cr-7
5-pyridoxic acid competitive Arthrobacter sp. Cr-7
DTNB 0.005 mM, 50% loss of activity after 1 h Arthrobacter sp. Cr-7
HgCl2 0.02 mM, 67% loss of activity after 1 h Arthrobacter sp. Cr-7
N-ethylmaleimide 2.5 mM, 71% loss of activity after 1 h Arthrobacter sp. Cr-7
NADH noncompetitive Arthrobacter sp. Cr-7
PCMB 0.005 mM, 75% loss of activity after 1 h Arthrobacter sp. Cr-7
Thionicotinamide adenine dinucleotide noncompetitive Arthrobacter sp. Cr-7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
NAD+ pH 8.0, 25°C Arthrobacter sp. Cr-7
0.04
-
Isopyridoxal pH 8.0, 25°C Arthrobacter sp. Cr-7

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
242000
-
gel filtration Arthrobacter sp. Cr-7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isopyridoxal + NAD+ + H2O Arthrobacter sp. Cr-7 the enzyme participates in the degradation of pyridoxine 5-pyridoxate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Cr-7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter sp. Cr-7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopyridoxal + NAD+ + H2O the enzyme participates in the degradation of pyridoxine Arthrobacter sp. Cr-7 5-pyridoxate + NADH + H+
-
?
isopyridoxal + NAD+ + H2O the enzyme also catalyses the activity of EC 1.1.1.416, isopyridoxal dehydrogenase (5-pyridoxolactone-forming) Arthrobacter sp. Cr-7 5-pyridoxate + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 60000, SDS-PAGE Arthrobacter sp. Cr-7

Synonyms

Synonyms Comment Organism
isopyridoxal dehydrogenase
-
Arthrobacter sp. Cr-7

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
Isopyridoxal pH 8.0, 25°C Arthrobacter sp. Cr-7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.6
-
Arthrobacter sp. Cr-7

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+ or thionicotinamide adenine dinucleotide Arthrobacter sp. Cr-7

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00065
-
4-deoxyisopyridoxal pH 8.0, 25°C Arthrobacter sp. Cr-7
0.13
-
Thionicotinamide adenine dinucleotide pH 8.0, 25°C Arthrobacter sp. Cr-7
0.25
-
NADH pH 8.0, 25°C Arthrobacter sp. Cr-7
0.7
-
5-pyridoxic acid pH 8.0, 25°C Arthrobacter sp. Cr-7

Expression

Organism Comment Expression
Arthrobacter sp. Cr-7 the enzyme is induced in response to growth of the organisms on pyridoxine up

General Information

General Information Comment Organism
metabolism the enzyme participates in the degradation of pyridoxine Arthrobacter sp. Cr-7