Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NIFI1,2 | a regulatory protein, inhibits nitrogenase by competing with Fe protein for binding to the MoFe protein, NifI1,2 inhibits ATP- and MoFe protein-dependent oxidation of the Fe protein, and NIFI1,2 binding prevents association of the two nitrogenase components, the inhibition is relieved by 2-oxoglutarate. NIFI1,2 is unable to bind to an AlF4-stabilized Fe protein-MoFe protein complex. Both nifI1 and nifI2 are required for regulation in vivo | Methanococcus maripaludis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | as part of the Fe protein in the MoFe protein, [4Fe-4S] clusters | Methanococcus maripaludis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanococcus maripaludis | both nifI1 and nifI2 are required for regulation in vivo | ? | - |
? | |
reduced ferredoxin + H+ + N2 + ATP + H2O | Methanococcus maripaludis | - |
oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus maripaludis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | reaction mechanism, overview | Methanococcus maripaludis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | both nifI1 and nifI2 are required for regulation in vivo | Methanococcus maripaludis | ? | - |
? | |
additional information | the enzyme performs acetylene reduction | Methanococcus maripaludis | ? | - |
? | |
reduced ferredoxin + H+ + N2 + ATP + H2O | - |
Methanococcus maripaludis | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, acetylene reduction | Methanococcus maripaludis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanococcus maripaludis | |
FeMo cofactor | - |
Methanococcus maripaludis |