Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | inhibits electron flow | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction kinetics for the isolated MoFe protein in reaction with cyanide with ligands tert-butylcyanide, butyl isocyanide, or imidazole, bound to the cofactor protein, cyanide binding kinetics, stopped-flow measurements | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | dependent on, the enzyme complex contains a molybdenum-iron protein, and a dimeric iron protein | Klebsiella pneumoniae | |
Molybdenum | dependent on, the enzyme complex contains a molybdenum-iron protein harboring the active site | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + H+ + N2 + ATP + H2O | Klebsiella pneumoniae | - |
oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
reduced ferredoxin + H+ + N2 + ATP + H2O | Klebsiella pneumoniae M5a1 | - |
oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae M5a1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the enzyme complex and isolation of the MoFe protein thereof | Klebsiella pneumoniae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | the active site is located on the MoFe protein, active site structure and substrate binding mechanism | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene + ? | - |
Klebsiella pneumoniae | ethylene + ? | - |
? | |
acetylene + ? | - |
Klebsiella pneumoniae M5a1 | ethylene + ? | - |
? | |
cyanide + ? | cyanide favors the molydenum for binding | Klebsiella pneumoniae | ? | - |
? | |
cyanide + ? | cyanide favors the molydenum for binding | Klebsiella pneumoniae M5a1 | ? | - |
? | |
cyclopropene + ? | - |
Klebsiella pneumoniae | ? | - |
? | |
cyclopropene + ? | - |
Klebsiella pneumoniae M5a1 | ? | - |
? | |
ethylene + ? | - |
Klebsiella pneumoniae | ? | - |
? | |
ethylene + ? | - |
Klebsiella pneumoniae M5a1 | ? | - |
? | |
methyl isocyanide + ? | - |
Klebsiella pneumoniae | ? | - |
? | |
N2O + ? | - |
Klebsiella pneumoniae | ? | - |
? | |
N3- + ? | - |
Klebsiella pneumoniae | ? | - |
? | |
reduced ferredoxin + H+ + N2 + ATP + H2O | - |
Klebsiella pneumoniae | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
reduced ferredoxin + H+ + N2 + ATP + H2O | - |
Klebsiella pneumoniae M5a1 | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Klebsiella pneumoniae | |
Ferredoxin | - |
Klebsiella pneumoniae | |
iron-molybdenum cofactor | - |
Klebsiella pneumoniae |