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Literature summary for 1.18.6.1 extracted from

  • Blanchard, C.Z.; Hales, B.J.
    Isolation of two forms of the nitrogenase VFe protein from Azotobacter vinelandii (1996), Biochemistry, 35, 472-478.
    View publication on PubMed
Search for more literature for 1.18.6.1

Metals/Ions

Metals/Ions Comment Organism Structure
Iron VFe protein form 1 is an incomplete form that contains only 1 cofactor and 1 [4Fe-4S] cluster with an additional [Fe4-S4]-like cluster Azotobacter vinelandii
Iron contains [4Fe4S] cluster Azotobacter vinelandii
additional information structure and organization of metal clusters Azotobacter vinelandii
Vanadium VFe protein form 1 is an incomplete form that contains only 1 cofactor and 1 [4Fe-4S] cluster with an additional [Fe4-S4]-like cluster Azotobacter vinelandii
Vanadium 2 forms of VFe protein: form 1 has V-toFe ratio of 1:19, form 2 of 1:15 Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii biological N2 fixation 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
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 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
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 2 forms of VFe protein
-

Purification (Commentary)

Purification (Comment) Organism
2 forms of VFe protein Azotobacter vinelandii

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate structure of V-containing enzyme form Azotobacter vinelandii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
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 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O biological N2 fixation Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Subunits

Subunits Comment Organism
More VFe protein form 1 has alphabeta2 conformation, VFe protein form 2 has alpha2beta2 conformation Azotobacter vinelandii