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Literature summary for 1.18.6.1 extracted from

  • Johnson, J.L.; Tolley, A.M.; Erickson, J.A.; Watt, G.D.
    Steady-state kinetic studies of dithionite utilization, component protein interaction, and the formation of an oxidized iron protein intermediate during Azotobacter vinelandii nitrogenase catalysis (1996), Biochemistry, 35, 11336-11342.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ required for MgATP complex Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
Fe protein Azotobacter vinelandii
230000
-
MoFe protein Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
dithionite + H+ + N2 + ATP in vitro substrate Azotobacter vinelandii ?
-
?
dithionite + H+ + N2 + ATP SO2- being the actual nitrogenase reductant, reaction kinetics Azotobacter vinelandii ?
-
?
reduced ferredoxin + H+ + ATP
-
Azotobacter vinelandii oxidized ferredoxin + H2 + ADP + phosphate
-
?