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Literature summary for 1.18.6.1 extracted from

  • Kim, J.; Rees, D.C.
    Nitrogenase and biological nitrogen fixation (1994), Biochemistry, 33, 389-397.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
homocitrate plays role in electron transfer at the [4Fe-4S] cluster to the MoFe-cofactor of the MoFe protein, can be substituted by erythro-fluorohomocitrate but not by threo-fluorohomocitrate Clostridium pasteurianum
homocitrate plays role in electron transfer at the [4Fe-4S] cluster to the MoFe-cofactor of the MoFe protein, can be substituted by erythro-fluorohomocitrate but not by threo-fluorohomocitrate Azotobacter vinelandii
homocitrate plays role in electron transfer at the [4Fe-4S] cluster to the MoFe-cofactor of the MoFe protein, can be substituted by erythro-fluorohomocitrate but not by threo-fluorohomocitrate Azotobacter chroococcum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains [4Fe4S] cluster Clostridium pasteurianum
Iron contains [4Fe4S] cluster Azotobacter vinelandii
Iron contains [4Fe4S] cluster Azotobacter chroococcum
Mg2+ Mg2+ required for MgATP complex Clostridium pasteurianum
Mg2+ Mg2+ required for MgATP complex Azotobacter vinelandii
Mg2+ Mg2+ required for MgATP complex Azotobacter chroococcum
Molybdenum MoFe-cofactor contains 2 clusters of composition [4Fe-3S] and [1Mo-3Fe-3S] that are brigded by 3 nonprotein ligands Clostridium pasteurianum
Molybdenum MoFe-cofactor contains 2 clusters of composition [4Fe-3S] and [1Mo-3Fe-3S] that are brigded by 3 nonprotein ligands Azotobacter vinelandii
Molybdenum MoFe-cofactor contains 2 clusters of composition [4Fe-3S] and [1Mo-3Fe-3S] that are brigded by 3 nonprotein ligands Azotobacter chroococcum
additional information structure and organization of metal clusters Clostridium pasteurianum
additional information structure and organization of metal clusters Azotobacter vinelandii
additional information structure and organization of metal clusters Azotobacter chroococcum
Vanadium characterization of the metal clusters in the nitrogenase vanadium-iron protein Clostridium pasteurianum
Vanadium characterization of the metal clusters in the nitrogenase vanadium-iron protein Azotobacter vinelandii
Vanadium characterization of the metal clusters in the nitrogenase vanadium-iron protein Azotobacter chroococcum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
Fe protein Clostridium pasteurianum
60000
-
Fe protein Azotobacter vinelandii
60000
-
Fe protein Azotobacter chroococcum
60000
-
about Clostridium pasteurianum
60000
-
about Azotobacter vinelandii
60000
-
about Azotobacter chroococcum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum biological N2 fixation 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii biological N2 fixation 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter chroococcum biological N2 fixation 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter chroococcum
-
nitrogen fixation complex is encoded on nif gene cluster
-
Azotobacter vinelandii
-
nitrogen fixation complex is encoded on nif gene cluster
-
Azotobacter vinelandii
-
2 forms of VFe protein
-
Clostridium pasteurianum
-
nitrogen fixation complex is encoded on nif gene cluster
-

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter chroococcum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
Clostridium pasteurianum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
Azotobacter chroococcum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O MgATP-dependent Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O MgATP-dependent Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O MgATP-dependent Azotobacter chroococcum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O biological N2 fixation Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O biological N2 fixation Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O biological N2 fixation Azotobacter chroococcum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
reduced ferredoxin + H+ + CN- + ATP
-
Clostridium pasteurianum oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
-
?
reduced ferredoxin + H+ + CN- + ATP
-
Azotobacter vinelandii oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
-
?
reduced ferredoxin + H+ + CN- + ATP
-
Azotobacter chroococcum oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
-
?
reduced ferredoxin + H+ + N3- + ATP
-
Clostridium pasteurianum oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
?
reduced ferredoxin + H+ + N3- + ATP
-
Azotobacter vinelandii oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
?
reduced ferredoxin + H+ + N3- + ATP
-
Azotobacter chroococcum oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
?

Cofactor

Cofactor Comment Organism Structure
additional information structure models of Fe protein, MoFe protein, and MoFe-cofactor, and their metal centers, e.g. the [4Fe-4S] cluster based on crystallographic data, substrate binding and electron transfer Clostridium pasteurianum
additional information structure models of Fe protein, MoFe protein, and MoFe-cofactor, and their metal centers, e.g. the [4Fe-4S] cluster based on crystallographic data, substrate binding and electron transfer Azotobacter vinelandii
additional information structure models of Fe protein, MoFe protein, and MoFe-cofactor, and their metal centers, e.g. the [4Fe-4S] cluster based on crystallographic data, substrate binding and electron transfer Azotobacter chroococcum