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Literature summary for 1.18.6.1 extracted from

  • Christiansen, J.; Cash, V.L.; Seefeldt, L.C.; Dean, D.R.
    Isolation and characterization of an acetylene-resistant nitrogenase (2000), J. Biol. Chem., 275, 11459-11464.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information
-
Azotobacter vinelandii
S69G alpha-subunit MoFe protein, resistant to inhibition by C2H2, thus acetylene binding/reduction site is not directly relevant to the mechanism of nitrogen reduction Azotobacter vinelandii

Inhibitors

Inhibitors Comment Organism Structure
C2H2 competitive to N2 Azotobacter vinelandii
CO
-
Azotobacter vinelandii
H2 competitive Azotobacter vinelandii
N2 inhibits the C2H2 reduction Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains [4Fe4S] cluster Azotobacter vinelandii
Molybdenum
-
Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O Azotobacter vinelandii
-
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate C2H2 inhibition mechanism, structure model Azotobacter vinelandii
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O active site for acetylene reduction interacts not directly with N2 reduction Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?