Protein Variants | Comment | Organism |
---|---|---|
additional information | - |
Azotobacter vinelandii |
S69G | alpha-subunit MoFe protein, resistant to inhibition by C2H2, thus acetylene binding/reduction site is not directly relevant to the mechanism of nitrogen reduction | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
C2H2 | competitive to N2 | Azotobacter vinelandii | |
CO | - |
Azotobacter vinelandii | |
H2 | competitive | Azotobacter vinelandii | |
N2 | inhibits the C2H2 reduction | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains [4Fe4S] cluster | Azotobacter vinelandii | |
Molybdenum | - |
Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | Azotobacter vinelandii | - |
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter vinelandii | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | C2H2 inhibition mechanism, structure model | Azotobacter vinelandii | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Azotobacter vinelandii | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | active site for acetylene reduction interacts not directly with N2 reduction | Azotobacter vinelandii | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |