Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SCN- | above 6 mM occurs substrate inhibition | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
SCN- | - |
Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ required for MgATP complex | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter vinelandii | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Azotobacter vinelandii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.27 | 2.28 | purified enzyme, substrate acetylene | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Azotobacter vinelandii | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
reduced ferredoxin + H+ + CN- + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + CH4 + NH3 + ADP + phosphate | - |
? | |
reduced ferredoxin + H+ + N3- + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + NH3 + N2 + ADP + phosphate | - |
? | |
reduced ferredoxin + H+ + SCN- + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + H2S + HCN + ADP + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
SCN- | - |
Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
SCN- reduction | Azotobacter vinelandii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10.1 | - |
SCN- | - |
Azotobacter vinelandii |