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Literature summary for 1.18.6.1 extracted from

  • Schneider, K.; Gollan, U.; Drottboom, M.; Selsemeier-Voigt, S.; Muller, A.
    Comparative biochemical characterization of the iron-only nitrogenase and the molybdenum nitrogenase from Rhodobacter capsulatus (1997), Eur. J. Biochem., 244, 789-800.
    View publication on PubMed
Search for more literature for 1.18.6.1

Protein Variants

Protein Variants Comment Organism
additional information construction of 2 mutants strain: 1 kanamycin-resistant with a deletion in NifHDK and 1 kanamycin, gentamycin, and molybdenum-resistant with double deletion in nif HDK and modABCD Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the iron-only enzyme consists of 2 components: Fe protein and FeFe protein, the latter contains 26 Fe atoms per molecul of protein Rhodobacter capsulatus
additional information the iron-only nitrogenase form contains no molybdenum, vanadium or any other heterometal atom Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13500
-
 FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE Rhodobacter capsulatus
51000
-
 FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE Rhodobacter capsulatus
57000
-
 MoFe protein: 2 * 59000 + 2 * 57000, alpha2beta2, SDS-PAGE Rhodobacter capsulatus
59000
-
 MoFe protein: 2 * 59000 + 2 * 57000, alpha2beta2, SDS-PAGE Rhodobacter capsulatus
59000
-
 FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodobacter capsulatus
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodobacter capsulatus B10S
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-
Rhodobacter capsulatus
-
 nitrogen fixation complex is encoded on nif gene cluster
-
Rhodobacter capsulatus
-
 iron-only nitrogenase and molybdenum nitrogenase
-
Rhodobacter capsulatus B10S
-
-
-

Purification (Commentary)

Purification (Comment) Organism
iron-only nitrogenase, both components Rhodobacter capsulatus

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate iron-only enzyme is composed of 2 components: FeFe protein and Fe protein Rhodobacter capsulatus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhodobacter capsulatus
0.235
-
 substrate N2, purified Mo nitrogenase Rhodobacter capsulatus
0.26
-
 substrate C2H2, iron-only nitrogenase with Fe protein to FeFe protein ratio of 40:1 Rhodobacter capsulatus
0.35
-
 substrate N2, iron-only nitrogenase with Fe protein to FeFe protein ratio of 40:1 Rhodobacter capsulatus
1.2
-
 substrate C2H2, purified Mo nitrogenase Rhodobacter capsulatus
1.3
-
 substrate H+, purified Mo nitrogenase Rhodobacter capsulatus
2.4
-
 substrate H+, iron-only nitrogenase with Fe protein to FeFe protein ratio of 40:1 Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Rhodobacter capsulatus 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Rhodobacter capsulatus B10S 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhodobacter capsulatus 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhodobacter capsulatus B10S 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
reduced ferredoxin + H+ + ATP H2-producing activity is much higher in the iron-only enzyme form than in the molybdenum containing form and is less inhibited by competitive substrates Rhodobacter capsulatus oxidized ferredoxin + H2 + ADP + phosphate
-
 ?
reduced ferredoxin + H+ + ATP H2-producing activity is much higher in the iron-only enzyme form than in the molybdenum containing form and is less inhibited by competitive substrates Rhodobacter capsulatus B10S oxidized ferredoxin + H2 + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? MoFe protein: 2 * 59000 + 2 * 57000, alpha2beta2, SDS-PAGE Rhodobacter capsulatus
? FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE Rhodobacter capsulatus
? Fe protein of both enzyme forms: 2 * 30000-31000, gamma2, SDS-PAGE Rhodobacter capsulatus
tetramer
-
 Rhodobacter capsulatus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 below and above no remaining activity Rhodobacter capsulatus