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Literature summary for 1.18.6.1 extracted from

  • Hallenbeck, P.C.; Meyer, C.; Vignais, P.M.
    Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata: purification and molecular properties (1982), J. Bacteriol., 149, 708-717.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information Fe protein contains an adenine-like molecule, a pentose moiety and a phosphate residue covalently attached to the molecule Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
Rhodobacter capsulatus
Molybdenum
-
Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
Rhodobacter capsulatus
33500
-
MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE Rhodobacter capsulatus
55000
-
MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE Rhodobacter capsulatus
59000
-
MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE Rhodobacter capsulatus
63000
-
Fe protein Rhodobacter capsulatus
230000
-
MoFe protein Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodobacter capsulatus
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodobacter capsulatus

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6 0.8 purified Fe protein Rhodobacter capsulatus
1.5 1.7 purified MoFe protein Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Rhodobacter capsulatus 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Subunits

Subunits Comment Organism
More
-
Rhodobacter capsulatus
tetramer MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE Rhodobacter capsulatus