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Literature summary for 1.18.6.1 extracted from

  • Yates, M.G.; Planque, K.
    Nitrogenase from Azotobacter chroococcum. Purification and properties of the component proteins (1975), Eur. J. Biochem., 60, 467-476.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 4 atoms of iron per molecule of Fe protein Azotobacter chroococcum
Molybdenum 23 mol Fe + 1.9 mol Mo per mol of MoFe protein Azotobacter chroococcum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
Azotobacter chroococcum
30800
-
MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE Azotobacter chroococcum
60000
-
MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE Azotobacter chroococcum
64000
-
Fe protein, gel filtration Azotobacter chroococcum
227000
-
MoFe protein, gel filtration Azotobacter chroococcum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter chroococcum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter chroococcum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large scale Azotobacter chroococcum

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter chroococcum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Azotobacter chroococcum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Subunits

Subunits Comment Organism
? MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE Azotobacter chroococcum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 MoFe protein stable Azotobacter chroococcum
8.7
-
50% loss of activity after overnight dialysis at pH 8.7 Azotobacter chroococcum