Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 4 atoms of iron per molecule of Fe protein | Azotobacter chroococcum | |
Molybdenum | 23 mol Fe + 1.9 mol Mo per mol of MoFe protein | Azotobacter chroococcum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Azotobacter chroococcum |
30800 | - |
MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE | Azotobacter chroococcum |
60000 | - |
MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE | Azotobacter chroococcum |
64000 | - |
Fe protein, gel filtration | Azotobacter chroococcum |
227000 | - |
MoFe protein, gel filtration | Azotobacter chroococcum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter chroococcum | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter chroococcum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
large scale | Azotobacter chroococcum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter chroococcum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter chroococcum | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE | Azotobacter chroococcum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | MoFe protein stable | Azotobacter chroococcum |
8.7 | - |
50% loss of activity after overnight dialysis at pH 8.7 | Azotobacter chroococcum |