Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.18.6.1 extracted from

  • Kush, A.; Elmerich, C.; Aubert, J.P.
    Nitrogenase of Sesbania Rhizobium strain ORS571: purification, properties, and 'switch-off' by ammonia (1985), J. Gen. Microbiol., 131, 1765-1777.
No PubMed abstract available

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 22.5 atoms of iron per molecule of protein Rhizobium sp.
Iron 3.1 Rhizobium sp.
Molybdenum 1.2-1.3 gatom per mol of MoFe protein Rhizobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, SDS-PAGE Rhizobium sp.
74000
-
Fe protein, gel filtration Rhizobium sp.
219000
-
MoFe protein, gel filtration Rhizobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhizobium sp.
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rhizobium sp.
-
ORS571, associated with Sesbania rostrata
-

Purification (Commentary)

Purification (Comment) Organism
ORS571 Rhizobium sp.

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhizobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Rhizobium sp. 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Rhizobium sp.
tetramer alpha: 56000, beta: 59000 Rhizobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhizobium sp.