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Literature summary for 1.18.6.1 extracted from

  • Hirschberg, R.; Samson, S.M.; Kimmel, B.E.; Page, K.A.; Collins, J.J.; Myers, J.A.; Yarbrough, L.R.
    Cloning and characterization of nitrogenase genes from Anabaena variabilis (1985), J. Biotechnol., 2, 23-37.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
amino acid sequence comparison with other species Trichormus variabilis
expression in Escherichia coli, expression in Klebsiella pneumoniae, Anabaena gene library screening with genes of Klebsiella pneumoniae Trichormus variabilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Trichormus variabilis
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
nitrogen fixation complex is encoded on nif gene cluster
-
Trichormus variabilis
-
nitrogen fixation complex is encoded on nif gene cluster
-

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Trichormus variabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Trichormus variabilis 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?