Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | - |
Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.2 gatom per mol of MoFe protein | Klebsiella pneumoniae | |
Cu2+ | 1.4 gatom per mol of MoFe protein | Klebsiella pneumoniae | |
Iron | 4 atoms of iron per molecule of Fe protein | Klebsiella pneumoniae | |
Iron | 17.5 gatom per mol of MoFe protein | Klebsiella pneumoniae | |
Mg2+ | 1.8 gatom per mol of MoFe protein | Klebsiella pneumoniae | |
Mg2+ | Mg2+ required for MgATP complex | Klebsiella pneumoniae | |
Molybdenum | 1 gatom per mol of MoFe protein | Klebsiella pneumoniae | |
Zn2+ | 0.8 gatom per mol of MoFe protein | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex | Klebsiella pneumoniae |
62000 | - |
Fe protein, gel filtration | Klebsiella pneumoniae |
220000 | - |
MoFe protein, gel filtration | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Klebsiella pneumoniae | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Oxidation Stability | Organism |
---|---|
extreme O2 lability, t1/2: 10 min, MoFe protein 45 s, Fe protein | Klebsiella pneumoniae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | catalytic mechanism | Klebsiella pneumoniae | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Klebsiella pneumoniae | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |