Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Castellaniella defragrans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
limonene | pH 8.0, 28°C | Castellaniella defragrans | |
0.043 | - |
limonene | pH 8.0, 40°C | Castellaniella defragrans | |
0.392 | - |
perillyl alcohol | pH 8.0, 28°C | Castellaniella defragrans | |
0.487 | - |
perillyl alcohol | pH 8.0, 40°C | Castellaniella defragrans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Castellaniella defragrans | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
152000 | - |
gel filtration | Castellaniella defragrans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-limonene + H2O + acceptor | Castellaniella defragrans | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | (+)-perillyl alcohol + reduced acceptor | - |
? | |
(R)-limonene + H2O + acceptor | Castellaniella defragrans 65Phen | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | (+)-perillyl alcohol + reduced acceptor | - |
? | |
(S)-limonene + H2O + acceptor | Castellaniella defragrans | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF). The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | (-)-perillyl alcohol + reduced acceptor | - |
? | |
(S)-limonene + H2O + acceptor | Castellaniella defragrans 65Phen | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF). The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | (-)-perillyl alcohol + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Castellaniella defragrans | W8X9R6 AND W8X092 | W8X9R6: CtmA, W8X092: CtmB | - |
Castellaniella defragrans 65Phen | W8X9R6 AND W8X092 | W8X9R6: CtmA, W8X092: CtmB | - |
Purification (Comment) | Organism |
---|---|
- |
Castellaniella defragrans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-p-menth-1-ene + H2O + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans | [(4S)-4-(propan-2-yl)cyclohex-1-en-1-yl]methanol + reduced ferrocenium hexafluorophosphate | - |
? | |
(+)-p-menth-1-ene + H2O + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans 65Phen | [(4S)-4-(propan-2-yl)cyclohex-1-en-1-yl]methanol + reduced ferrocenium hexafluorophosphate | - |
? | |
(R)-(-)-alpha-phellandrene + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans | ? + reduced ferrocenium hexafluorophosphate | - |
? | |
(R)-(-)-alpha-phellandrene + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans 65Phen | ? + reduced ferrocenium hexafluorophosphate | - |
? | |
(R)-limonene + H2O + acceptor | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | Castellaniella defragrans | (+)-perillyl alcohol + reduced acceptor | - |
? | |
(R)-limonene + H2O + acceptor | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | Castellaniella defragrans 65Phen | (+)-perillyl alcohol + reduced acceptor | - |
? | |
(R)-limonene + H2O + ferrocenium hexafluorophosphate | hydroxylates the R- and S-enantiomers at a similar rate | Castellaniella defragrans | (+)-perillyl alcohol + reduced ferrocenium hexafluorophosphate | - |
? | |
(S)-limonene + H2O + acceptor | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF). The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | Castellaniella defragrans | (-)-perillyl alcohol + reduced acceptor | - |
? | |
(S)-limonene + H2O + acceptor | the in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF). The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF) | Castellaniella defragrans 65Phen | (-)-perillyl alcohol + reduced acceptor | - |
? | |
(S)-limonene + H2O + ferrocenium hexafluorophosphate | hydroxylates the R- and S-enantiomers at a similar rate | Castellaniella defragrans | (-)-perillyl alcohol + reduced ferrocenium hexafluorophosphate | - |
? | |
alpha-terpinene + ferrocenium hexafluorophosphate | - |
Castellaniella defragrans | ? + reduced ferrocenium hexafluorophosphate | - |
? | |
limonene + H2O + ferrocenium hexafluorophosphate | hydroxylates the R- and S-enantiomers at a similar rate | Castellaniella defragrans | perillyl alcohol + reduced ferrocenium hexafluorophosphate | - |
? | |
additional information | the native enzyme (CtmAB) oxidizes a wide range of monocyclic monoterpenes containing the allylic methyl group motif (1-methyl-cyclohex-1-ene) | Castellaniella defragrans | ? | - |
? | |
additional information | the native enzyme (CtmAB) oxidizes a wide range of monocyclic monoterpenes containing the allylic methyl group motif (1-methyl-cyclohex-1-ene) | Castellaniella defragrans 65Phen | ? | - |
? | |
perillyl alcohol + dithiothreitol | - |
Castellaniella defragrans | limonene + H2O + dithiothreitol S-oxide | - |
? | |
terpinolene + ferrocenium hexafluorophosphate | low activity | Castellaniella defragrans | ? + reduced ferrocenium hexafluorophosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 60700 (CtmB) + 1 * 61700 (CtmA), SDS-PAGE | Castellaniella defragrans |
Synonyms | Comment | Organism |
---|---|---|
ctmAB | - |
Castellaniella defragrans |
limonene:ferrocenium oxidoreductase | - |
Castellaniella defragrans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Castellaniella defragrans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | potassium phosphate buffer | Castellaniella defragrans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | each subunit (CtmA and CtmB) carries a flavin cofactor | Castellaniella defragrans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
perillyl alcohol | pH 8.0, 28°C | Castellaniella defragrans | |
0.0103 | - |
perillyl alcohol | pH 8.0, 40°C | Castellaniella defragrans | |
47 | - |
limonene | pH 8.0, 28°C | Castellaniella defragrans | |
63 | - |
limonene | pH 8.0, 40°C | Castellaniella defragrans |