Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | the enzyme is quickly inactivated in air in its reduced state | Azoarcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Azoarcus sp. | |
0.0018 | - |
3-ethylpyridine | pH 7.5, 30°C | Azoarcus sp. | |
0.0023 | - |
2-ethylpyrrole | pH 7.5, 30°C | Azoarcus sp. | |
0.0052 | - |
2-Ethylphenol | pH 7.5, 30°C | Azoarcus sp. | |
0.0093 | - |
3-Ethylphenol | pH 7.5, 30°C | Azoarcus sp. | |
0.0243 | - |
2-ethylaniline | pH 7.5, 30°C | Azoarcus sp. | |
0.0247 | - |
2-ethylfuran | pH 7.5, 30°C | Azoarcus sp. | |
0.0283 | - |
ferricenium | pH 7.5, 30°C | Azoarcus sp. | |
0.0405 | - |
4-Ethylphenol | pH 7.5, 30°C | Azoarcus sp. | |
0.045 | - |
ethylbenzene | pH 7.5, 30°C | Azoarcus sp. | |
0.0665 | - |
2-ethylthiophene | pH 7.5, 30°C | Azoarcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a molybdenum/iron-sulfur/heme protein | Azoarcus sp. | |
Molybdenum | a molybdenum/iron-sulfur/heme protein | Azoarcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
- |
Azoarcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethylbenzene + H2O + acceptor | Azoarcus sp. | - |
(S)-1-phenylethanol + reduced acceptor | - |
? | |
ethylbenzene + H2O + acceptor | Azoarcus sp. EbN1 | - |
(S)-1-phenylethanol + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azoarcus sp. | - |
i.e. Aromatoleum aromaticum | - |
Azoarcus sp. EbN1 | - |
i.e. Aromatoleum aromaticum | - |
Oxidation Stability | Organism |
---|---|
the enzyme is quickly inactivated in air in its reduced state | Azoarcus sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ethylbenzene + H2O + acceptor = (S)-1-phenylethanol + reduced acceptor | kinetics and mechanism of oxygen-independent hydrocarbon hydroxylation, quantitative structure-activity relationship modeling | Azoarcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-diethylbenzene + H2O + ferricenium | - |
Azoarcus sp. | 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocenium | - |
? | |
1,4-diethylbenzene + H2O + ferricenium | - |
Azoarcus sp. EbN1 | 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocenium | - |
? | |
2-ethylaniline + H2O + ferricenium | - |
Azoarcus sp. | ? | - |
? | |
2-ethylaniline + H2O + ferricenium | - |
Azoarcus sp. EbN1 | ? | - |
? | |
2-ethylfuran + H2O + ferricenium | - |
Azoarcus sp. | 1-(2-furyl)ethanol + ferrocenium | - |
? | |
2-ethylfuran + H2O + ferricenium | - |
Azoarcus sp. | ? | - |
? | |
2-ethylphenol + H2O + ferricenium | - |
Azoarcus sp. | 2-(1-hydroxyethyl)phenol + ferrocenium | - |
? | |
2-ethylpyrrole + H2O + ferricenium | - |
Azoarcus sp. | ? | - |
? | |
2-ethylthiophene + H2O + ferricenium | - |
Azoarcus sp. | 1-thien-2-ylethanol + ferrocenium | - |
? | |
2-ethylthiophene + H2O + ferricenium | - |
Azoarcus sp. | ? | - |
? | |
3-ethylphenol + H2O + ferricenium | - |
Azoarcus sp. | 3-(1-hydroxyethyl)phenol + ferrocenium | - |
? | |
3-ethylpyridine + H2O + ferricenium | - |
Azoarcus sp. | ? | - |
? | |
4-ethylanisole + H2O + ferricenium | - |
Azoarcus sp. | 1-(4-methoxyphenyl)ethanol + ferrocenium | - |
? | |
4-ethylbiphenyl + H2O + ferricenium | - |
Azoarcus sp. | 1-(1,1'-biphenyl-4-yl)ethanol + reduced ferrocenium | - |
? | |
4-ethylbiphenyl + H2O + ferricenium | - |
Azoarcus sp. EbN1 | 1-(1,1'-biphenyl-4-yl)ethanol + reduced ferrocenium | - |
? | |
4-ethylphenol + H2O + ferricenium | best substrate | Azoarcus sp. | 4-(1-hydroxyethyl)phenol + ferrocenium | - |
? | |
4-ethyltoluene + H2O + ferricenium | - |
Azoarcus sp. | 1-(4-methylphenyl)ethanol + ferrocenium | - |
? | |
4-propylphenol + H2O + ferricenium | - |
Azoarcus sp. | 4-(1-hydroxypropyl)phenol + ferrocenium | - |
? | |
ethylbenzene + H2O + acceptor | - |
Azoarcus sp. | (S)-1-phenylethanol + reduced acceptor | - |
? | |
ethylbenzene + H2O + acceptor | - |
Azoarcus sp. EbN1 | (S)-1-phenylethanol + reduced acceptor | - |
? | |
ethylbenzene + H2O + ferricenium | EBDH catalyzes the oxygen-independent, stereospecific hydroxylation of ethylbenzene to (S)-1-phenylethanol, the direct anaerobic oxidation of a nonactivated hydrocarbon | Azoarcus sp. | (S)-1-phenylethanol + ferrocenium | - |
? | |
ethylidenecyclohexane + H2O + ferricenium | - |
Azoarcus sp. | ? | - |
? | |
additional information | substrate specificity, product identification by GC- and LC-MS, overview | Azoarcus sp. | ? | - |
? | |
additional information | substrate specificity, product identification by GC- and LC-MS, overview | Azoarcus sp. EbN1 | ? | - |
? | |
propylbenzene + H2O + ferricenium | - |
Azoarcus sp. | (S)-1-phenylpropanol + ferrocenium | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | quantitative structure-activity relationship modeling | Azoarcus sp. |
trimer | - |
Azoarcus sp. |
Synonyms | Comment | Organism |
---|---|---|
EBDH | - |
Azoarcus sp. |
ethylbenzene dehydrogenase | - |
Azoarcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Azoarcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | relative kcat values | Azoarcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Azoarcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | a molybdenum/iron-sulfur/heme protein | Azoarcus sp. |