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Literature summary for 1.17.99.2 extracted from

  • Szaleniec, M.; Hagel, C.; Menke, M.; Nowak, P.; Witko, M.; Heider, J.
    Kinetics and mechanism of oxygen-independent hydrocarbon hydroxylation by ethylbenzene dehydrogenase (2007), Biochemistry, 46, 7637-7646.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
O2 the enzyme is quickly inactivated in air in its reduced state Azoarcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Azoarcus sp.
0.0018
-
3-ethylpyridine pH 7.5, 30°C Azoarcus sp.
0.0023
-
2-ethylpyrrole pH 7.5, 30°C Azoarcus sp.
0.0052
-
2-Ethylphenol pH 7.5, 30°C Azoarcus sp.
0.0093
-
3-Ethylphenol pH 7.5, 30°C Azoarcus sp.
0.0243
-
2-ethylaniline pH 7.5, 30°C Azoarcus sp.
0.0247
-
2-ethylfuran pH 7.5, 30°C Azoarcus sp.
0.0283
-
ferricenium pH 7.5, 30°C Azoarcus sp.
0.0405
-
4-Ethylphenol pH 7.5, 30°C Azoarcus sp.
0.045
-
ethylbenzene pH 7.5, 30°C Azoarcus sp.
0.0665
-
2-ethylthiophene pH 7.5, 30°C Azoarcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a molybdenum/iron-sulfur/heme protein Azoarcus sp.
Molybdenum a molybdenum/iron-sulfur/heme protein Azoarcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
-
Azoarcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethylbenzene + H2O + acceptor Azoarcus sp.
-
(S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + acceptor Azoarcus sp. EbN1
-
(S)-1-phenylethanol + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Azoarcus sp.
-
i.e. Aromatoleum aromaticum
-
Azoarcus sp. EbN1
-
i.e. Aromatoleum aromaticum
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is quickly inactivated in air in its reduced state Azoarcus sp.

Reaction

Reaction Comment Organism Reaction ID
ethylbenzene + H2O + acceptor = (S)-1-phenylethanol + reduced acceptor kinetics and mechanism of oxygen-independent hydrocarbon hydroxylation, quantitative structure-activity relationship modeling Azoarcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-diethylbenzene + H2O + ferricenium
-
Azoarcus sp. 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocenium
-
?
1,4-diethylbenzene + H2O + ferricenium
-
Azoarcus sp. EbN1 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocenium
-
?
2-ethylaniline + H2O + ferricenium
-
Azoarcus sp. ?
-
?
2-ethylaniline + H2O + ferricenium
-
Azoarcus sp. EbN1 ?
-
?
2-ethylfuran + H2O + ferricenium
-
Azoarcus sp. 1-(2-furyl)ethanol + ferrocenium
-
?
2-ethylfuran + H2O + ferricenium
-
Azoarcus sp. ?
-
?
2-ethylphenol + H2O + ferricenium
-
Azoarcus sp. 2-(1-hydroxyethyl)phenol + ferrocenium
-
?
2-ethylpyrrole + H2O + ferricenium
-
Azoarcus sp. ?
-
?
2-ethylthiophene + H2O + ferricenium
-
Azoarcus sp. 1-thien-2-ylethanol + ferrocenium
-
?
2-ethylthiophene + H2O + ferricenium
-
Azoarcus sp. ?
-
?
3-ethylphenol + H2O + ferricenium
-
Azoarcus sp. 3-(1-hydroxyethyl)phenol + ferrocenium
-
?
3-ethylpyridine + H2O + ferricenium
-
Azoarcus sp. ?
-
?
4-ethylanisole + H2O + ferricenium
-
Azoarcus sp. 1-(4-methoxyphenyl)ethanol + ferrocenium
-
?
4-ethylbiphenyl + H2O + ferricenium
-
Azoarcus sp. 1-(1,1'-biphenyl-4-yl)ethanol + reduced ferrocenium
-
?
4-ethylbiphenyl + H2O + ferricenium
-
Azoarcus sp. EbN1 1-(1,1'-biphenyl-4-yl)ethanol + reduced ferrocenium
-
?
4-ethylphenol + H2O + ferricenium best substrate Azoarcus sp. 4-(1-hydroxyethyl)phenol + ferrocenium
-
?
4-ethyltoluene + H2O + ferricenium
-
Azoarcus sp. 1-(4-methylphenyl)ethanol + ferrocenium
-
?
4-propylphenol + H2O + ferricenium
-
Azoarcus sp. 4-(1-hydroxypropyl)phenol + ferrocenium
-
?
ethylbenzene + H2O + acceptor
-
Azoarcus sp. (S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + acceptor
-
Azoarcus sp. EbN1 (S)-1-phenylethanol + reduced acceptor
-
?
ethylbenzene + H2O + ferricenium EBDH catalyzes the oxygen-independent, stereospecific hydroxylation of ethylbenzene to (S)-1-phenylethanol, the direct anaerobic oxidation of a nonactivated hydrocarbon Azoarcus sp. (S)-1-phenylethanol + ferrocenium
-
?
ethylidenecyclohexane + H2O + ferricenium
-
Azoarcus sp. ?
-
?
additional information substrate specificity, product identification by GC- and LC-MS, overview Azoarcus sp. ?
-
?
additional information substrate specificity, product identification by GC- and LC-MS, overview Azoarcus sp. EbN1 ?
-
?
propylbenzene + H2O + ferricenium
-
Azoarcus sp. (S)-1-phenylpropanol + ferrocenium
-
?

Subunits

Subunits Comment Organism
More quantitative structure-activity relationship modeling Azoarcus sp.
trimer
-
Azoarcus sp.

Synonyms

Synonyms Comment Organism
EBDH
-
Azoarcus sp.
ethylbenzene dehydrogenase
-
Azoarcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Azoarcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information relative kcat values Azoarcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Azoarcus sp.

Cofactor

Cofactor Comment Organism Structure
heme a molybdenum/iron-sulfur/heme protein Azoarcus sp.