Literature summary for 1.17.99.10 extracted from

Dermer, J.; Fuchs, G.
Molybdoenzyme that catalyzes the anaerobic hydroxylation of a tertiary carbon atom in the side chain of cholesterol (2012), J. Biol. Chem., 287, 36905-36916 .

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mo2+	molybdoenzyme	Sterolibacterium denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cholest-4-en-3-one + acceptor + H2O	Sterolibacterium denitrificans	-	25-hydroxycholest-4-en-3-one + reduced acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Sterolibacterium denitrificans	H9NN89 AND H9NN90 AND H9NN91	H9NN89: steroid C25 dehydrogenase alpha subunit, H9NN90: steroid C25 dehydrogenase beta subunit, H9NN91: steroid C25 dehydrogenase gamma subunit	-

Oxidation Stability

Oxidation Stability	Organism
purified enzyme is inactivated irreversibly by incubation in air with a half-life of 14 min. The inactivation is largely prevented by the addition of the artificial electron acceptor potassium hexacyanoferrate (III) to the enzyme preparation. In the presence of cyanoferrate, more than 90% of the enzyme activity is retained after a 2 h incubation in air. The enzymatic activity is completely lost after 24 h of incubation	Sterolibacterium denitrificans

Organism

purified enzyme is inactivated irreversibly by incubation in air with a half-life of 14 min. The inactivation is largely prevented by the addition of the artificial electron acceptor potassium hexacyanoferrate (III) to the enzyme preparation. In the presence of cyanoferrate, more than 90% of the enzyme activity is retained after a 2 h incubation in air. The enzymatic activity is completely lost after 24 h of incubation

Sterolibacterium denitrificans

Storage Stability

Storage Stability	Organism
the enzyme can be stored for at least 3 months at 4 °C under anaerobic conditions	Sterolibacterium denitrificans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
5-cholesten-3beta-ol-7-one + acceptor + H2O	15% of the activity as compared to activity with cholest-4-en-3-one	Sterolibacterium denitrificans	25-hydroxy-5-cholesten-3beta-ol-7-one + reduced acceptor	-	?
cholest-4-en-3-one + acceptor + H2O	-	Sterolibacterium denitrificans	25-hydroxycholest-4-en-3-one + reduced acceptor	-	?
cholesta-4,6-dien-3-one + acceptor + H2O	127% of the activity as compared to activity with cholest-4-en-3-one	Sterolibacterium denitrificans	25-hydroxycholesta-4,6-dien-3-one + reduced acceptor	-	?
cholesterol + acceptor + H2O	10% of the activity as compared to activity with cholest-4-en-3-one	Sterolibacterium denitrificans	25-hydroxycholesterol + reduced acceptor	-	?

Synonyms

Synonyms	Comment	Organism
C25 dehydrogenase	-	Sterolibacterium denitrificans