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Literature summary for 1.17.99.10 extracted from

  • Rugor, A.; Tataruch, M.; Staron, J.; Dudzik, A.; Niedzialkowska, E.; Nowak, P.; Hogendorf, A.; Michalik-Zym, A.; Napruszewska, D.; Jarzebski, A.; Szymanska, K.; Bialas, W.; Szaleniec, M.
    Regioselective hydroxylation of cholecalciferol, cholesterol and other sterol derivatives by steroid C25 dehydrogenase (2017), Appl. Microbiol. Biotechnol., 101, 1163-1174 .
    View publication on PubMed
Search for more literature for 1.17.99.10

General Stability

General Stability Organism
immobilization of the enzyme either on powder or a monolithic silica support functionalized with an organic linker providing NH2 groups for enzyme covalent binding extends the catalytic lifetime of the enzyme under working conditions at least 3.5times Sterolibacterium denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Mo2+ molybdenum enzyme Sterolibacterium denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholecalciferol + acceptor + H2O Sterolibacterium denitrificans the product 25-hydroxycholecalciferol (calcifediol) is the activated form of vitamin D3 used in the treatment of rickets and other calcium disorders 25-hydroxycholecalciferol + reduced acceptor
-
 ?
cholesterol + acceptor + H2O Sterolibacterium denitrificans the product 25-hydroxycholesterol is responsible for a complex regulatory function in the immunological system 25-hydroxycholesterol + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Sterolibacterium denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sterolibacterium denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 immobilization of the enzyme either on powder or a monolithic silica support functionalized with an organic linker providing NH2 groups for enzyme covalent binding reduces the initial activity of the enzyme more than twofold Sterolibacterium denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholecalciferol + acceptor + H2O the product 25-hydroxycholecalciferol (calcifediol) is the activated form of vitamin D3 used in the treatment of rickets and other calcium disorders Sterolibacterium denitrificans 25-hydroxycholecalciferol + reduced acceptor
-
 ?
cholecalciferol + acceptor + H2O 90% concversion after 46 h Sterolibacterium denitrificans 25-hydroxycholecalciferol + reduced acceptor
-
 ?
cholest-4-en-3-one + acceptor + H2O 90% concversion after 4 h Sterolibacterium denitrificans 25-hydroxycholest-4-en-3-one + reduced acceptor
-
 ?
cholesta-1,4-dien-3-one + acceptor + H2O 90% concversion after 4 h Sterolibacterium denitrificans 25-hydroxycholesta-1,4-dien-3-one + reduced acceptor
-
 ?
cholesterol + acceptor + H2O
-
 Sterolibacterium denitrificans 25-hydroxycholesterol + reduced acceptor
-
 ?
cholesterol + acceptor + H2O the product 25-hydroxycholesterol is responsible for a complex regulatory function in the immunological system Sterolibacterium denitrificans 25-hydroxycholesterol + reduced acceptor
-
 ?

Synonyms

Synonyms Comment Organism
S25DH
-
 Sterolibacterium denitrificans
steroid C25 dehydrogenase
-
 Sterolibacterium denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sterolibacterium denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Sterolibacterium denitrificans