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Literature summary for 1.17.9.1 extracted from

  • Efimov, I.; McIntire, W.S.
    Relationship between charge-transfer interactions, redox potentials, and catalysis for different forms of the flavoprotein component of p-cresol methylhydroxylase (2005), J. Am. Chem. Soc., 127, 732-741.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9200
-
alpha, beta, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida
58700
-
alpha, beta, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-cresol + acceptor + H2O Pseudomonas putida via intermediate 4-hydroxybenzylalcohol 4-hydroxybenzaldehyde + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-cresol + acceptor + H2O via intermediate 4-hydroxybenzylalcohol Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
?

Subunits

Subunits Comment Organism
tetramer alpha, beta, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
4-Cresol 2’-deoxy-FAD cofactor, pH 7, 25°C Pseudomonas putida
121
-
4-Cresol FAD cofactor, pH 7, 25°C Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
2'-deoxy-FAD strong binding but significantly reduced activity Pseudomonas putida
FAD
-
Pseudomonas putida
additional information only traces of activity with 6-Br-FAD, iso-FAD, 5-deaza-FAD, 6-amino-FAD, or 6-hydroxy-FAD as cofactor Pseudomonas putida