Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
R474K | reduced rate of FAD binding, strongly reduced activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9200 | - |
alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
59500 | - |
alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-cresol + acceptor + H2O | Pseudomonas putida | via intermediate 4-hydroxybenzylalcohol | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
4-cresol + acceptor + H2O | Pseudomonas putida NCIMB 9869 | via intermediate 4-hydroxybenzylalcohol | 4-hydroxybenzaldehyde + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
NCIMB 9869 | - |
Pseudomonas putida NCIMB 9869 | - |
NCIMB 9869 | - |
Purification (Comment) | Organism |
---|---|
native and mutant enzymes from Escherichia coli | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-cresol + acceptor + H2O | via intermediate 4-hydroxybenzylalcohol | Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
4-cresol + acceptor + H2O | via intermediate 4-hydroxybenzylalcohol | Pseudomonas putida NCIMB 9869 | 4-hydroxybenzaldehyde + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
4-Cresol | R474K mutant enzyme, pH 7, 25°C | Pseudomonas putida | |
121 | - |
4-Cresol | native enzyme, pH 7, 25°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas putida |