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Literature summary for 1.17.9.1 extracted from

  • Efimov, I.; Cronin, C.N.; Bergmann, D.J.; Kuusk, V.; McIntire, W.S.
    Insight into covalent flavinylation and catalysis from redox, spectral, and kinetic analyses of the R474K mutant of the flavoprotein subunit of p-cresol methylhydroxylase (2004), Biochemistry, 43, 6138-6148.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
R474K reduced rate of FAD binding, strongly reduced activity Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9200
-
alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida
59500
-
alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-cresol + acceptor + H2O Pseudomonas putida via intermediate 4-hydroxybenzylalcohol 4-hydroxybenzaldehyde + reduced acceptor
-
?
4-cresol + acceptor + H2O Pseudomonas putida NCIMB 9869 via intermediate 4-hydroxybenzylalcohol 4-hydroxybenzaldehyde + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
NCIMB 9869
-
Pseudomonas putida NCIMB 9869
-
NCIMB 9869
-

Purification (Commentary)

Purification (Comment) Organism
native and mutant enzymes from Escherichia coli Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-cresol + acceptor + H2O via intermediate 4-hydroxybenzylalcohol Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
?
4-cresol + acceptor + H2O via intermediate 4-hydroxybenzylalcohol Pseudomonas putida NCIMB 9869 4-hydroxybenzaldehyde + reduced acceptor
-
?

Subunits

Subunits Comment Organism
tetramer alpha, beta, 2 * 59500 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
4-Cresol R474K mutant enzyme, pH 7, 25°C Pseudomonas putida
121
-
4-Cresol native enzyme, pH 7, 25°C Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas putida