Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | weak inhibition of the enzyme, when FAD is used as reducing cofactor | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+ and Mn2+, at 0.5 mM, lower the content of isopentenyl diphosphate in the mixture of both products, isopentenyl diphosphate and dimethylallyl diphosphate | Escherichia coli | |
Fe2+ | enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster | Escherichia coli | |
Mn2+ | activates | Escherichia coli | |
Mn2+ | Co2+ and Mn2+, at 0.5 mM, lower the content of isopentenyl diphosphate in the mixture of both products, isopentenyl diphosphate and dimethylallyl diphosphate | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36591 | - |
2 * 36591, recombinant His-tagged enzyme, amino acid sequence determination | Escherichia coli |
72000 | - |
recombinant His-tagged enzyme, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Escherichia coli | - |
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | Escherichia coli | part of isoprenoid biosynthesis | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | Escherichia coli | 2-C-methyl-D-erithritol-4-phosphate pathway | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene ispH, enzyme LytB | - |
Oxidation Stability | Organism |
---|---|
enzyme possesses a O2-sensitive [4Fe-4S] cluster | Escherichia coli |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged apoprotein from Escherichia coli strain M15, to homogeneity | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
reconstitution of purified apoenzyme with prosthetic group [Fe4-S4] cluster | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
purified apoenzyme, with FAD and NADPH as reducing cofactors | Escherichia coli |
0.011 | - |
purified apoenzyme, with photoreduced deazaflavin as reducing cofactor | Escherichia coli |
0.03 | - |
reconstituted purified enzyme, with photoreduced deazaflavin as reducing cofactor | Escherichia coli |
0.044 | - |
reconstituted purified enzyme, with FAD and NADPH as reducing cofactors | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Escherichia coli | isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | Co2+ and Mn2+ lower the content of isopentenyl diphosphate in the mixture of both products, isopentenyl diphosphate and dimethylallyl diphosphate | r | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Escherichia coli | dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | part of isoprenoid biosynthesis | Escherichia coli | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | 2-C-methyl-D-erithritol-4-phosphate pathway | Escherichia coli | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36591, recombinant His-tagged enzyme, amino acid sequence determination | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
deazaflavin | requires a reducing system, e.g. flavodoxin/flavodoxin reductase or NADPH or the photoreduced deazaflavin radical | Escherichia coli | |
FAD | requires a reducing system, e.g. flavodoxin/flavodoxin reductase or NADPH or the photoreduced deazaflavin radical | Escherichia coli | |
additional information | enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster as prosthetic group | Escherichia coli | |
NADP+ | - |
Escherichia coli | |
NADPH | requires a reducing system, e.g. flavodoxin/flavodoxin reductase or NADPH or the photoreduced deazaflavin radical | Escherichia coli |