Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, 25% (w/v) polyethylene glycol 3350, 3% (v/v) ethylene glycol | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D237A | inactive | Arabidopsis thaliana |
E262A | the mutant loses its catalytic activity, probably for its impaired FAD binding capacity | Arabidopsis thaliana |
H417A | the mutant formes inclusion bodies during expression, and hence its activity is not measureable | Arabidopsis thaliana |
H417N | inactive | Arabidopsis thaliana |
H417Y | inactive | Arabidopsis thaliana |
Q332A | the mutant aggregates and its activity is not measureable | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
195000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
71-hydroxychlorophyll a + reduced ferredoxin + H+ | Arabidopsis thaliana | - |
chlorophyll a + oxidized ferredoxin + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8GS60 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
71-hydroxychlorophyll a + reduced ferredoxin + H+ | - |
Arabidopsis thaliana | chlorophyll a + oxidized ferredoxin + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 49000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
HCAR | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana | |
[4Fe-4S]-center | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is required for the degradation of light-harvesting complexes and is necessary for efficient photosynthesis by balancing the chlorophyll a/b ratio | Arabidopsis thaliana |