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Literature summary for 1.17.4.2 extracted from

  • Blakley, R.L.
    Ribonucleoside triphosphate reductase from Lactobacillus leichmannii (1978), Methods Enzymol., 51, 246-259.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dATP specific activator for CTP reduction Lactobacillus leichmannii
dCTP specific activator for UTP reduction Lactobacillus leichmannii
dGTP specific activator for ATP reduction Lactobacillus leichmannii
dTTP specific activator for ITP reduction Lactobacillus leichmannii

General Stability

General Stability Organism
fairly stable under conditions of storage, losing only about 23% of its activity in 15 months Lactobacillus leichmannii

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyadenosylcobalamin
-
Lactobacillus leichmannii
3-isoadenosylcobalamin
-
Lactobacillus leichmannii
adenosylcobalamin
-
Lactobacillus leichmannii
isopropylideneadenosylcobalamin
-
Lactobacillus leichmannii
nebularylcobalamin
-
Lactobacillus leichmannii
tubercidylcobalamin
-
Lactobacillus leichmannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
adenosylcobalamin presence of 1.0 mM CTP and 1 mM dATP Lactobacillus leichmannii
0.00045
-
adenosylcobalamin presence of 0.1 mM CTP and 1 mM dATP Lactobacillus leichmannii
0.0055
-
adenosylcobalamin presence of 2 mM CTP without activator Lactobacillus leichmannii
0.13
-
CTP presence of 1 mM dATP Lactobacillus leichmannii
0.22
-
ATP presence of 1 mM dGTP Lactobacillus leichmannii

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ activating effect Lactobacillus leichmannii
K+ activating effect Lactobacillus leichmannii
Li+ activating effect Lactobacillus leichmannii
Na+ activating effect Lactobacillus leichmannii
NH4+ activating effect Lactobacillus leichmannii
Rb+ activating effect Lactobacillus leichmannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
-
Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleoside triphosphate + reduced thioredoxin + H2O Pseudomonas sp.
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Corynebacterium sp.
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Clostridium sp.
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Rhizobium sp.
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Euglena sp.
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Astasia sp.
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r

Organism

Organism UniProt Comment Textmining
Astasia sp.
-
euglenoid flagellate
-
Clostridium sp.
-
-
-
Corynebacterium sp.
-
-
-
Euglena sp.
-
euglenoid flagellate
-
Lactobacillus leichmannii
-
strain ATCC 4797
-
Pseudomonas sp.
-
-
-
Rhizobium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.82
-
-
Lactobacillus leichmannii

Storage Stability

Storage Stability Organism
-100°C, may be stored as frozen paste for at least 3 months without significant loss of activity Lactobacillus leichmannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + reduced thioredoxin
-
Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + dihydrolipoate
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + lipoate + H2O
-
?
ribonucleoside triphosphate + dithioerythritol
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + ? + H2O
-
?
ribonucleoside triphosphate + dithiothreitol
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + ? + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Pseudomonas sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Corynebacterium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Clostridium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Rhizobium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Euglena sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Astasia sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Pseudomonas sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Corynebacterium sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Clostridium sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Rhizobium sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Euglena sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Lactobacillus leichmannii deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Astasia sp. deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Pseudomonas sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Corynebacterium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Clostridium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Rhizobium sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Euglena sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Astasia sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r

Subunits

Subunits Comment Organism
monomer
-
Lactobacillus leichmannii

Cofactor

Cofactor Comment Organism Structure
Coalpha-(aden-9-yl)-Cobeta-adenosylcobamide
-
Lactobacillus leichmannii
Coalpha-(benzimidazolyl)-Cobeta-adenosylcobamide
-
Lactobacillus leichmannii
coenzyme B12 adenosylcobalamin Pseudomonas sp.
coenzyme B12 adenosylcobalamin Corynebacterium sp.
coenzyme B12 adenosylcobalamin Clostridium sp.
coenzyme B12 adenosylcobalamin Rhizobium sp.
coenzyme B12 adenosylcobalamin Euglena sp.
coenzyme B12 adenosylcobalamin Lactobacillus leichmannii
coenzyme B12 adenosylcobalamin Astasia sp.