Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dATP | specific activator for CTP reduction | Lactobacillus leichmannii | |
dCTP | specific activator for UTP reduction | Lactobacillus leichmannii | |
dGTP | specific activator for ATP reduction | Lactobacillus leichmannii | |
dTTP | specific activator for ITP reduction | Lactobacillus leichmannii |
General Stability | Organism |
---|---|
fairly stable under conditions of storage, losing only about 23% of its activity in 15 months | Lactobacillus leichmannii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyadenosylcobalamin | - |
Lactobacillus leichmannii | |
3-isoadenosylcobalamin | - |
Lactobacillus leichmannii | |
adenosylcobalamin | - |
Lactobacillus leichmannii | |
isopropylideneadenosylcobalamin | - |
Lactobacillus leichmannii | |
nebularylcobalamin | - |
Lactobacillus leichmannii | |
tubercidylcobalamin | - |
Lactobacillus leichmannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
adenosylcobalamin | presence of 1.0 mM CTP and 1 mM dATP | Lactobacillus leichmannii | |
0.00045 | - |
adenosylcobalamin | presence of 0.1 mM CTP and 1 mM dATP | Lactobacillus leichmannii | |
0.0055 | - |
adenosylcobalamin | presence of 2 mM CTP without activator | Lactobacillus leichmannii | |
0.13 | - |
CTP | presence of 1 mM dATP | Lactobacillus leichmannii | |
0.22 | - |
ATP | presence of 1 mM dGTP | Lactobacillus leichmannii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | activating effect | Lactobacillus leichmannii | |
K+ | activating effect | Lactobacillus leichmannii | |
Li+ | activating effect | Lactobacillus leichmannii | |
Na+ | activating effect | Lactobacillus leichmannii | |
NH4+ | activating effect | Lactobacillus leichmannii | |
Rb+ | activating effect | Lactobacillus leichmannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
- |
Lactobacillus leichmannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside triphosphate + reduced thioredoxin + H2O | Pseudomonas sp. | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Corynebacterium sp. | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Clostridium sp. | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Rhizobium sp. | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Euglena sp. | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Lactobacillus leichmannii | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Astasia sp. | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Astasia sp. | - |
euglenoid flagellate | - |
Clostridium sp. | - |
- |
- |
Corynebacterium sp. | - |
- |
- |
Euglena sp. | - |
euglenoid flagellate | - |
Lactobacillus leichmannii | - |
strain ATCC 4797 | - |
Pseudomonas sp. | - |
- |
- |
Rhizobium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus leichmannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.82 | - |
- |
Lactobacillus leichmannii |
Storage Stability | Organism |
---|---|
-100°C, may be stored as frozen paste for at least 3 months without significant loss of activity | Lactobacillus leichmannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + reduced thioredoxin | - |
Lactobacillus leichmannii | dATP + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + dihydrolipoate | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + lipoate + H2O | - |
? | |
ribonucleoside triphosphate + dithioerythritol | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + ? + H2O | - |
? | |
ribonucleoside triphosphate + dithiothreitol | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + ? + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Pseudomonas sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Corynebacterium sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Clostridium sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Rhizobium sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Euglena sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Astasia sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Pseudomonas sp. | deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Corynebacterium sp. | deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Clostridium sp. | deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Rhizobium sp. | deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Euglena sp. | deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Astasia sp. | deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Pseudomonas sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Corynebacterium sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Clostridium sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Rhizobium sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Euglena sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Astasia sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Lactobacillus leichmannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Coalpha-(aden-9-yl)-Cobeta-adenosylcobamide | - |
Lactobacillus leichmannii | |
Coalpha-(benzimidazolyl)-Cobeta-adenosylcobamide | - |
Lactobacillus leichmannii | |
coenzyme B12 | adenosylcobalamin | Pseudomonas sp. | |
coenzyme B12 | adenosylcobalamin | Corynebacterium sp. | |
coenzyme B12 | adenosylcobalamin | Clostridium sp. | |
coenzyme B12 | adenosylcobalamin | Rhizobium sp. | |
coenzyme B12 | adenosylcobalamin | Euglena sp. | |
coenzyme B12 | adenosylcobalamin | Lactobacillus leichmannii | |
coenzyme B12 | adenosylcobalamin | Astasia sp. |