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Literature summary for 1.17.4.2 extracted from

  • Hamilton, F.D.
    Ribonucleotide reductase from Euglena gracilis. A 5-deoxyadenoslycobalamin-dependent enzyme (1974), J. Biol. Chem., 249, 4428-4434.
    View publication on PubMed
Search for more literature for 1.17.4.2

Activating Compound

Activating Compound Comment Organism Structure
5'-deoxyadenosylcobalamin
-
 Euglena gracilis
5'-deoxyadenosylcobalamin
-
 Lactobacillus leichmannii
ATP stimulates CTP reduction Euglena gracilis
dATP specific activator for CTP reduction Euglena gracilis
dATP specific activator for CTP reduction Lactobacillus leichmannii
dCTP
-
 Euglena gracilis
dCTP
-
 Lactobacillus leichmannii
dGTP
-
 Euglena gracilis
dGTP
-
 Lactobacillus leichmannii
dTTP
-
 Euglena gracilis
dTTP
-
 Lactobacillus leichmannii

Inhibitors

Inhibitors Comment Organism Structure
dTTP inhibits CTP, ATP and GTP reduction, reduction of UTP is unaffected by deoxyribonucleotides Euglena gracilis
dTTP
-
 Lactobacillus leichmannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 CTP in presence of dATP Euglena gracilis
0.15
-
 CTP in presence of dTTP Euglena gracilis
0.25
-
 ATP in presence of dTTP Euglena gracilis
0.44
-
 ATP
-
 Euglena gracilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli
Mg2+ can stimulate the reaction Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 4 * 100000, SDS-PAGE Euglena gracilis
140000 150000
-
 Euglena gracilis
240000 278000
-
 Escherichia coli
440000
-
 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleoside triphosphate + reduced thioredoxin + H2O Escherichia coli
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O Euglena gracilis
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Euglena gracilis
-
-
-
Euglena gracilis Z
-
-
-
Lactobacillus leichmannii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Euglena gracilis
-
 Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.167
-
-
 Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + reduced thioredoxin
-
 Escherichia coli dADP + oxidized thioredoxin + H2O
-
 ?
ADP + reduced thioredoxin
-
 Lactobacillus leichmannii dADP + oxidized thioredoxin + H2O
-
 ?
ATP + reduced thioredoxin
-
 Euglena gracilis dATP + oxidized thioredoxin + H2O
-
 ?
ATP + reduced thioredoxin
-
 Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
 ?
ATP + reduced thioredoxin
-
 Euglena gracilis Z dATP + oxidized thioredoxin + H2O
-
 ?
CDP + reduced thioredoxin
-
 Escherichia coli dCDP + oxidized thioredoxin + H2O
-
 ?
CDP + reduced thioredoxin
-
 Lactobacillus leichmannii dCDP + oxidized thioredoxin + H2O
-
 ?
CTP + reduced thioredoxin
-
 Euglena gracilis dCTP + oxidized thioredoxin + H2O
-
 ?
CTP + reduced thioredoxin
-
 Lactobacillus leichmannii dCTP + oxidized thioredoxin + H2O
-
 ?
CTP + reduced thioredoxin
-
 Euglena gracilis Z dCTP + oxidized thioredoxin + H2O
-
 ?
GTP + reduced thioredoxin
-
 Euglena gracilis dGTP + oxidized thioredoxin + H2O
-
 ?
GTP + reduced thioredoxin
-
 Lactobacillus leichmannii dGTP + oxidized thioredoxin + H2O
-
 ?
GTP + reduced thioredoxin
-
 Euglena gracilis Z dGTP + oxidized thioredoxin + H2O
-
 ?
ribonucleoside triphosphate + reduced thioredoxin
-
 Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin
-
 Euglena gracilis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin
-
 Euglena gracilis Z 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Euglena gracilis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
UTP + reduced thioredoxin
-
 Euglena gracilis dUTP + oxidized thioredoxin + H2O
-
 ?
UTP + reduced thioredoxin
-
 Lactobacillus leichmannii dUTP + oxidized thioredoxin + H2O
-
 ?
UTP + reduced thioredoxin
-
 Euglena gracilis Z dUTP + oxidized thioredoxin + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 100000, SDS-PAGE Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
NADPH can utilize NADPH as hydrogen donor for ribonucleotide reduction Euglena gracilis