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Literature summary for 1.17.4.2 extracted from

  • Goulian, M.; Beck, W.S.
    Purification and properties of cobamide-dependent ribonucleotide reductase from Lactobacillus leichmannii (1966), J. Biol. Chem., 241, 4233-4242.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dATP
-
Lactobacillus leichmannii
dGTP specific activator for ATP reduction Lactobacillus leichmannii
NADPH stimulates reductase activity in crude extracts Lactobacillus leichmannii

Inhibitors

Inhibitors Comment Organism Structure
dTTP inhibits ATP reduction Lactobacillus leichmannii
Mg2+ strongly inhibitory in absence of ATP Lactobacillus leichmannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
CTP
-
Lactobacillus leichmannii
2.3
-
ATP
-
Lactobacillus leichmannii
9.5
-
CTP without ATP Lactobacillus leichmannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
sedimentation equilibrium analysis Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii physiological hydrogen donor is unknown 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r

Organism

Organism UniProt Comment Textmining
Lactobacillus leichmannii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
576
-
-
Lactobacillus leichmannii

Storage Stability

Storage Stability Organism
-20°C, crude extracts relatively unstable, most of the activity disappears within a few weeks Lactobacillus leichmannii
0-4°C, acetone and hydroxylapatite fractions are quite stable, at least 70-80% of their activities remain after 3 months Lactobacillus leichmannii
0-4°C, crude extracts relatively unstable, most of the activity disappears within a few weeks Lactobacillus leichmannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + reduced thioredoxin most active diphosphate substrate Lactobacillus leichmannii dADP + oxidized thioredoxin + H2O
-
?
ATP + reduced thioredoxin
-
Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
?
CDP + reduced thioredoxin poorly reduced Lactobacillus leichmannii dCDP + oxidized thioredoxin + H2O
-
?
CMP + reduced thioredoxin poorly reduced Lactobacillus leichmannii dCMP + oxidized thioredoxin + H2O
-
?
CTP + reduced thioredoxin
-
Lactobacillus leichmannii dCTP + oxidized thioredoxin + H2O
-
?
GDP + reduced thioredoxin poorly reduced Lactobacillus leichmannii dGDP + oxidized thioredoxin + H2O
-
?
GTP + reduced thioredoxin
-
Lactobacillus leichmannii dGTP + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O physiological hydrogen donor is unknown Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
UDP + reduced thioredoxin poorly reduced Lactobacillus leichmannii dUDP + oxidized thioredoxin + H2O
-
?
UTP + reduced thioredoxin
-
Lactobacillus leichmannii dUTP + oxidized thioredoxin + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
5,6-dimethylbenzimidazolycobamide
-
Lactobacillus leichmannii
coenzyme B12
-
Lactobacillus leichmannii
dihydrolipoate
-
Lactobacillus leichmannii