Literature summary for 1.17.3.2 extracted from

Pauff, J.M.; Zhang, J.; Bell, C.E.; Hille, R.
Substrate orientation in xanthine oxidase: crystal structure of enzyme in reaction with 2-hydroxy-6-methylpurine (2008), J. Biol. Chem., 283, 4818-4824.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with inhibitor 2-hydroxy-6-methylpurine, 10 mg/ml purified enzyme in 40 mM Tris-HCl, pH 7.8, 20 mM diphosphate, pH 8.5, 0.2 mM EDTA and 5 mM DTT, batch method mixing 0.02 ml of protein solution and 0.01 ml of precipitant solution, the latter containing of 12-14% PEG 8000, 0.1 M potassium phosphate, and 0.2 mM EDTA, pH 7.2, for 2-3 days at 25°C, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement	Bos taurus

Crystallization (Comment)

Organism

enzyme in complex with inhibitor 2-hydroxy-6-methylpurine, 10 mg/ml purified enzyme in 40 mM Tris-HCl, pH 7.8, 20 mM diphosphate, pH 8.5, 0.2 mM EDTA and 5 mM DTT, batch method mixing 0.02 ml of protein solution and 0.01 ml of precipitant solution, the latter containing of 12-14% PEG 8000, 0.1 M potassium phosphate, and 0.2 mM EDTA, pH 7.2, for 2-3 days at 25°C, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement

Bos taurus

Inhibitors

Inhibitors	Comment	Organism
2-hydroxy-6-methylpurine	interacts with Arg880 in both active sites of the enzyme dimer, binding structure, overview	Bos taurus
alloxanthine	-	Bos taurus
FYX-051	-	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Molybdenum	active site molybdenum center structure, overview	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
xanthine + H2O + O2	Bos taurus	-	urate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P80457	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme partially by gel filtration from fresh unpasteurized milk	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
milk	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
xanthine + H2O + O2	-	Bos taurus	urate + H2O2	-	?
xanthine + H2O + O2	orientation of xanthine in the active site of xanthine oxidoreductase,structure, overview	Bos taurus	urate + H2O2	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Bos taurus