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Literature summary for 1.17.3.2 extracted from

  • Kalra, S.; Jena, G.; Tikoo, K.; Mukhopadhyay, A.K.
    Preferential inhibition of xanthine oxidase by 2-amino-6-hydroxy-8-mercaptopurine and 2-amino-6-purine thiol (2007), BMC Biochem., 8, 8.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.3.2

Inhibitors

Inhibitors Comment Organism Structure
2-amino-6-hydroxy-8-mercaptopurine mixed-type inhibition, the inhibitor specifically blocks the enzyme activity with the drug 6-mercaptopurine, but does affect activity with xanthine and hypoxanthine to a lesser extent, overview Bos taurus
2-amino-6-purine thiol competitive inhibitor, the inhibitor specifically blocks the enzyme activity with the drug 6-mercaptopurine, but does affect activity with xanthine and hypoxanthine to a lesser extent, overview Bos taurus
allopurinol a suicide inhibitor of XOD, a pyrazolopyrimidine derivative, and an analogue of hypoxanthine Bos taurus
additional information substrate-dependent inhibitory potencies, overview Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-mercaptopurine + H2O + O2 Bos taurus an anticancer drug ?
-
 ?
hypoxanthine + 2 H2O + 2 O2 Bos taurus
-
 urate + 2 H2O2
-
 ?
xanthine + H2O + O2 Bos taurus from dead cancer cells urate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-mercaptopurine + 2 H2O + 2 O2 production of superoxide radicals Bos taurus 6-thiouric acid + 2 H2O2
-
 ?
6-mercaptopurine + H2O + O2 an anticancer drug Bos taurus ?
-
 ?
hypoxanthine + 2 H2O + 2 O2
-
 Bos taurus urate + 2 H2O2
-
 ?
hypoxanthine + 2 H2O + 2 O2 via intermediate xanthine formation, production of superoxide radicals Bos taurus urate + 2 H2O2
-
 ?
xanthine + H2O + O2 from dead cancer cells Bos taurus urate + H2O2
-
 ?
xanthine + H2O + O2 production of superoxide radicals Bos taurus urate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
XOD
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Bos taurus
0.00096
-
 2-amino-6-hydroxy-8-mercaptopurine pH 7.4, competitive versus substrate 6-mercaptopurine Bos taurus
0.00098
-
 2-amino-6-hydroxy-8-mercaptopurine pH 7.4, noncompetitive versus substrate 6-mercaptopurine Bos taurus
0.0013
-
 2-amino-6-purine thiol pH 7.4, competitive versus substrate 6-mercaptopurine Bos taurus
0.0057
-
 2-amino-6-hydroxy-8-mercaptopurine pH 7.4, competitive versus substrate xanthine Bos taurus
0.00624
-
 2-amino-6-hydroxy-8-mercaptopurine pH 7.4, noncompetitive versus substrate xanthine Bos taurus
0.0066
-
 2-amino-6-purine thiol pH 7.4, competitive versus substrate xanthine Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 in vitro bi-substrate-inhibitor-enzyme simulation and kinetics, overview Bos taurus additional information
0.00054
-
 pH 7.4, versus substrate 6-mercaptopurine Bos taurus 2-amino-6-hydroxy-8-mercaptopurine
0.00091
-
 pH 7.4, versus substrate hypoxanthine Bos taurus allopurinol
0.00192
-
 pH 7.4, versus substrate 6-mercaptopurine Bos taurus allopurinol
0.00236
-
 pH 7.4, versus substrate xanthine Bos taurus allopurinol
0.0026
-
 pH 7.4, versus substrate 6-mercaptopurine Bos taurus 2-amino-6-purine thiol
0.0051
-
 pH 7.4, versus substrate hypoxanthine Bos taurus 2-amino-6-hydroxy-8-mercaptopurine
0.0073
-
 pH 7.4, versus substrate hypoxanthine Bos taurus 2-amino-6-purine thiol
0.0164
-
 pH 7.4, versus substrate xanthine Bos taurus 2-amino-6-purine thiol
0.0177
-
 pH 7.4, versus substrate xanthine Bos taurus 2-amino-6-hydroxy-8-mercaptopurine