Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | heme-containing inactive protein in inclusion bodies is renatured and reactivated with pyrroloquinoline quinone and Ca2+ to give active enzyme | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72256 | - |
1 * 72256, calculated from sequence | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lupanine + pyrroloquinoline quinone + H2O | Pseudomonas sp. | lupanine is a quinolizidine alkaloid produced by plants of the genus Lupinus. It is metabolized by a Pseudomonas sp. with conversion into 17-hydroxylupanine as the first step. The enzyme involved is induced by growth of the organism on lupanine. cofactors. It catalyses the dehydrogenation of lupanine with the PQQ acting as electron acceptor and the electrons are transferred to the haem before being passed to an external acceptor. This acceptor has not yet been identified but the enzyme rapidly reduces horse heart cytochrome c. Addition of water to the dehydrogenated lupanine gives the hydroxylated product | 17-hydroxylupanine + pyrroloquinoline quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q934G0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas sp. |
Renatured (Comment) | Organism |
---|---|
heme-containing inactive protein in inclusion bodies is renatured and reactivated with pyrroloquinoline quinone and Ca2+ to give active enzyme | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:lupanine-grown cell | - |
Pseudomonas sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lupanine + pyrroloquinoline quinone + H2O | - |
Pseudomonas sp. | 17-hydroxylupanine + pyrroloquinoline quinol | - |
? | |
lupanine + pyrroloquinoline quinone + H2O | lupanine is a quinolizidine alkaloid produced by plants of the genus Lupinus. It is metabolized by a Pseudomonas sp. with conversion into 17-hydroxylupanine as the first step. The enzyme involved is induced by growth of the organism on lupanine. cofactors. It catalyses the dehydrogenation of lupanine with the PQQ acting as electron acceptor and the electrons are transferred to the haem before being passed to an external acceptor. This acceptor has not yet been identified but the enzyme rapidly reduces horse heart cytochrome c. Addition of water to the dehydrogenated lupanine gives the hydroxylated product | Pseudomonas sp. | 17-hydroxylupanine + pyrroloquinoline quinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72256, calculated from sequence | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Pseudomonas sp. | |
pyrroloquinoline quinone | - |
Pseudomonas sp. |