Cloned (Comment) | Organism |
---|---|
gene VvXDH, sequence comparisons and phylogenetic analysis, synthesis of gene VvXDH through PCR-based two-step DNA synthesis method, recombinant overexpression in Arabidopsis thaliana ecotype Columbia under control of CaMV 35S promoter from plasmid pYM817 using the Agrobacterium tumefaciens strain GV3101 transfection method. The transgenic Arabidopsis thaliana plants show enhanced salt tolerance, phenotype, overview. Explicit function of VvXDH in conferring salt stress by increasing allantoin accumulation and activating abscisic acid signaling pathway, enhancing ROS scavenging in transgenic Arabidopsis thaliana. Expression of VvXDH results in elevated allantoin accumulation | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | Vitis vinifera | - |
urate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | F6GU50 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | - |
Vitis vinifera | urate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VvXDH | - |
Vitis vinifera |
XDH | - |
Vitis vinifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Vitis vinifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Vitis vinifera |
General Information | Comment | Organism |
---|---|---|
physiological function | xanthine dehydrogenase plays an important role in purine catabolism catalyzing the oxidative hydroxylation of hypoxanthine to xanthine and of xanthine to uric acid, it plays a role in recycling and remobilization of nitrogen, and XDH is implicated in plant stress responses and acclimation. Explicit function of VvXDH in conferring salt stress by increasing allantoin accumulation and activating abscisic acid signaling pathway, enhancing ROS scavenging in transgenic Arabidopsis thaliana. VvXDH application results in increased tolerance to abiotic stresses by elevating allantoin accumulation | Vitis vinifera |