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Literature summary for 1.17.1.4 extracted from

  • Boland, M.J.; Blevins, D.G.; Randall, D.D.
    Soybean nodule xanthine dehydrogenase: A kinetic study (1983), Arch. Biochem. Biophys., 222, 435-441.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5,5-dithiobis-(2-nitrobenzoate) 30% inhibition at 1 mM, presence of NAD+, no conversion from dehydrogenase to oxidase activity detectable Glycine max
diethyl dicarbonate 90% loss of NAD+ dependent activity at 1 mM, retains more than 90% of oxygen-dependent and 3-acetylpyridine adenine dinucleotide+-dependent NADH oxidation activity Glycine max
NADH varied substrate: xanthine, product inhibition, Ki 0.05 mM, varied substrate: NAD+, dead-end inhibition type, Ki 0.022 mM Glycine max
Urate varied substrate: xanthine, dead-end inhibition type, Ki 0.18 mM, varied substrate: NAD+, product inhibition, Ki 0.45 mM Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
xanthine at pH 7.5, ping-pong-reaction mechanism, strongly pH-dependent Glycine max
0.0063
-
NADH at pH 7.0, Tris-maleate buffer Glycine max
0.011
-
NADH at pH 8.0, Tris-maleate buffer Glycine max
0.0125
-
NAD+ xanthine oxidation at pH 7.5 Glycine max
0.02
-
NAD+ at pH 7.5, hypoxanthine oxidation Glycine max
0.021
-
3-acetylpyridine-adenine dinucleotide xanthine oxidation Glycine max
0.047 0.079 hypoxanthine measured in the pH range from 6 to 8.9, pH-independent Km Glycine max
0.102
-
3-acetylpyridine adenine dinucleotide+ NADH oxidation Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
325000
-
gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NAD+ + H+ + O2- + H2O Glycine max predominant reaction xanthine + NADH + H2O2
-
?
xanthine + NAD+ + H2O Glycine max
-
urate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
nodule
-
Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + NAD+ + H+ + O2- + H2O predominant reaction Glycine max xanthine + NADH + H2O2
-
?
xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O same activity compared to NAD+ Glycine max urate + 3-acetylpyridine-adenine dinucleotide(H)
-
r
xanthine + NAD+ + H2O
-
Glycine max urate + NADH
-
r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Glycine max
NADH
-
Glycine max