Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5-dithiobis-(2-nitrobenzoate) | 30% inhibition at 1 mM, presence of NAD+, no conversion from dehydrogenase to oxidase activity detectable | Glycine max | |
diethyl dicarbonate | 90% loss of NAD+ dependent activity at 1 mM, retains more than 90% of oxygen-dependent and 3-acetylpyridine adenine dinucleotide+-dependent NADH oxidation activity | Glycine max | |
NADH | varied substrate: xanthine, product inhibition, Ki 0.05 mM, varied substrate: NAD+, dead-end inhibition type, Ki 0.022 mM | Glycine max | |
Urate | varied substrate: xanthine, dead-end inhibition type, Ki 0.18 mM, varied substrate: NAD+, product inhibition, Ki 0.45 mM | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
xanthine | at pH 7.5, ping-pong-reaction mechanism, strongly pH-dependent | Glycine max | |
0.0063 | - |
NADH | at pH 7.0, Tris-maleate buffer | Glycine max | |
0.011 | - |
NADH | at pH 8.0, Tris-maleate buffer | Glycine max | |
0.0125 | - |
NAD+ | xanthine oxidation at pH 7.5 | Glycine max | |
0.02 | - |
NAD+ | at pH 7.5, hypoxanthine oxidation | Glycine max | |
0.021 | - |
3-acetylpyridine-adenine dinucleotide | xanthine oxidation | Glycine max | |
0.047 | 0.079 | hypoxanthine | measured in the pH range from 6 to 8.9, pH-independent Km | Glycine max | |
0.102 | - |
3-acetylpyridine adenine dinucleotide+ | NADH oxidation | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
325000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H+ + O2- + H2O | Glycine max | predominant reaction | xanthine + NADH + H2O2 | - |
? | |
xanthine + NAD+ + H2O | Glycine max | - |
urate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nodule | - |
Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H+ + O2- + H2O | predominant reaction | Glycine max | xanthine + NADH + H2O2 | - |
? | |
xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O | same activity compared to NAD+ | Glycine max | urate + 3-acetylpyridine-adenine dinucleotide(H) | - |
r | |
xanthine + NAD+ + H2O | - |
Glycine max | urate + NADH | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Glycine max | |
NADH | - |
Glycine max |