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Literature summary for 1.17.1.4 extracted from

  • Aretz, W.; Kaspari, H.; Klemme, J.H.
    Molecular and kinetic characterization of xanthine dehydrogenase from the phototrophic bacterium Rodopseudomonas capsulata (1981), Z. Naturforsch. C, 36, 933-941.
No PubMed abstract available
Search for more literature for 1.17.1.4

Inhibitors

Inhibitors Comment Organism Structure
1-methylhypoxanthine 17% inhibition of xanthine dehydrogenase at 0.25 mM Rhodobacter capsulatus
8-Azaadenine complete inhibition of xanthine dehydrogenase at 0.2 mM Rhodobacter capsulatus
8-Azaguanine complete inhibition of xanthine dehydrogenase at 0.2 mM Rhodobacter capsulatus
8-azohypoxanthine 40% inhibition of xanthine dehydrogenase at 0.25 mM Rhodobacter capsulatus
adenine competitive inhibition, Ki: 0.05 mM Rhodobacter capsulatus
Urate competitive inhibition of xanthine dehydrogenase, Ki: 0.144 mM Rhodobacter capsulatus
xanthine 40% inhibition of xanthine dehydrogenase at 0.25 mM Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0325
-
 xanthine
-
 Rhodobacter capsulatus
0.0525
-
 hypoxanthine
-
 Rhodobacter capsulatus
0.0612
-
 NAD+
-
 Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 8 atoms iron per mol enzyme Rhodobacter capsulatus
Mo 2 molybdenum per mol enzyme Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84200
-
 4 * 84200, SDS-PAGE Rhodobacter capsulatus
345000
-
 gel filtration Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NAD+ + H+ + O2- + H2O Rhodobacter capsulatus preferred substrate xanthine + NADH + H2O2
-
 ir
xanthine + NAD+ + H2O Rhodobacter capsulatus 67% of the activity compared to hypoxanthine urate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, affinity chromatography Rhodobacter capsulatus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible Rhodobacter capsulatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.9
-
-
 Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methylhypoxanthine + NAD+ + H2O 10% of the activity compared to hypoxanthine Rhodobacter capsulatus 1-methylxanthine + NADH
-
 ?
1-methylxanthine + NAD+ + H2O 10% of the activity compared to hypoxanthine Rhodobacter capsulatus 1-methylurate + NADH
-
 ?
2-hydroxypurine + NAD+ + H2O 35% of the activity compared to hypoxanthine, purine not oxidized Rhodobacter capsulatus ? + NADH
-
 ?
6,8-dihydropurine + NAD+ + H2O 50% of the activity compared to hypoxanthine Rhodobacter capsulatus ? + NADH
-
 ?
8-azahypoxanthine + NAD+ + H2O 39% 0f the activity compared to hypoxanthine Rhodobacter capsulatus 8-azaxanthine + NADH
-
 ?
hypoxanthine + NAD+ + H+ + O2- + H2O preferred substrate Rhodobacter capsulatus xanthine + NADH + H2O2
-
 ir
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Rhodobacter capsulatus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 r
xanthine + NAD+ + H2O 67% of the activity compared to hypoxanthine Rhodobacter capsulatus urate + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 84200, SDS-PAGE Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 highest activity, but unstable Rhodobacter capsulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable for 4 min Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
additional information
-
 Rhodobacter capsulatus
NAD+ cannot be replaced by NADP+ Rhodobacter capsulatus
NADH
-
 Rhodobacter capsulatus