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Literature summary for 1.17.1.4 extracted from
- Xanthine dehydrogenase and pteridine metabolism in Colias butterflies (1972), J. Biol. Chem., 247, 1445-1451.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Amino-2,6-dihydroxypyrimidine | competitive inhibition of xanthine oxidation, Ki: 0.106 mM | Colias eurytheme |  |
| Leucopterin | competitive inhibition of xanthopterin oxidation, Ki: 0.0109 mM | Colias eurytheme | |
| Urate | competitive inhibition of xanthine oxidation, Ki: 0.064 mM | Colias eurytheme | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
Xanthopterin | cosubstrate: NAD+ | Colias eurytheme | |
| 0.0061 | - |
2-amino-4-hydroxypterin | body preparation, cosubstrate: NAD+ | Colias eurytheme | |
| 0.0071 | - |
adenine | cosubstrate: NAD+ | Colias eurytheme | |
| 0.0072 | - |
2-amino-4-hydroxypterin | wing preparation, co substrate: NAD+ | Colias eurytheme | |
| 0.0182 | - |
purine | cosubstrate: NAD+ | Colias eurytheme | |
| 0.0258 | - |
4-hydroxypyrazolo(3,4-d)pyrimidine | cosubstrate: NAD+ | Colias eurytheme | |
| 0.0451 | - |
hypoxanthine | co substrate: NAD+ | Colias eurytheme | |
| 0.061 | - |
xanthine | wing preparation, cosubstrate: NAD+ | Colias eurytheme | |
| 0.0647 | - |
xanthine | body preparation, cosubstrate: NAD+ | Colias eurytheme | |
| 0.1065 | - |
NAD+ | cosubstrate: hypoxanthine | Colias eurytheme | |
| 0.124 | - |
NAD+ | cosubstrate: xanthine | Colias eurytheme | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 230000 | 300000 | gel filtration | Colias eurytheme |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-amino-4-hydroxy-pterin + NAD+ + H2O | Colias eurytheme | i.e. pterin | isoxanthopterin + NADH | - |
? | |
| hypoxanthine + NAD+ + H2O | Colias eurytheme | - |
xanthine + NADH + H+ | - |
? | |
| purine + NAD+ + H2O | Colias eurytheme | - |
? + NADH | - |
? | |
| xanthine + NAD+ + H2O | Colias eurytheme | - |
urate + NADH | - |
? | |
| xanthopterin + NAD+ + H2O | Colias eurytheme | regulation of the pteridine pathway by competitive inhibition of reaction products and the precursor of xanthopterin, 7,8-dihydroxanthopterin | leucopterin + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colias eurytheme | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Colias eurytheme |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| wing | - |
Colias eurytheme | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 1°C, 1 mM DTT in conjunction with 0.1 M ammonium sulfate, 60-70% retaining activity after 8-12 days | Colias eurytheme |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Colias eurytheme | isoxanthopterin + NADH | - |
? | |
| 2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Colias eurytheme | isoxanthopterin + NADH | - |
? | |
| 4-hydroxypyrazolo(3,4-d)pyrimidine + NAD+ + H2O | best substrate tested | Colias eurytheme | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + NADH | - |
? | |
| 4-hydroxypyrazolo(3,4-d)pyrimidine + NAD+ + H2O | i.e. allopurinol | Colias eurytheme | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + NADH | - |
? | |
| adenine + NAD+ + H2O | low activity | Colias eurytheme | ? + NADH | - |
? | |
| hypoxanthine + NAD+ + H2O | - |
Colias eurytheme | xanthine + NADH + H+ | - |
? | |
| purine + NAD+ + H2O | - |
Colias eurytheme | ? + NADH | - |
? | |
| xanthine + NAD+ + H2O | - |
Colias eurytheme | urate + NADH | - |
? | |
| xanthopterin + NAD+ + H2O | regulation of the pteridine pathway by competitive inhibition of reaction products and the precursor of xanthopterin, 7,8-dihydroxyxanthopterin | Colias eurytheme | leucopterin + NADH | - |
? | |
| xanthopterin + NAD+ + H2O | regulation of the pteridine pathway by competitive inhibition of reaction products and the precursor of xanthopterin, 7,8-dihydroxanthopterin | Colias eurytheme | leucopterin + NADH | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
at 30°C | Colias eurytheme |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | stable within, loss of activity at more acidic pHs | Colias eurytheme |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Colias eurytheme | |
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